Promising bactericidal approach of dihydrazone analogues against bio-film forming Gram-negative bacteria and molecular mechanistic studies

K. P. Rakesh, H. K. Vivek, H. M. Manukumar, C. S. Shantharam, Bukhari Syed Nasir Abbas, Hua Li Qin, M. B. Sridhara

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Gram-negative members of the ESCAPE family are more difficult to treat, due to the presence of an additional barrier in the form of a lipopolysaccharide layer and the efficiency of efflux pumps to pump out the drugs from the cytoplasm. The development of alternative therapeutic strategies to tackle ESCAPE Gram-negative members is of extreme necessity to provide a solution to the cause of life-threatening infections. The present investigations demonstrated that compounds 17, 20, 25 and 26 possessing the presence of electron donating (OH and OCH3) groups on the phenyl rings are highly potent; whereas compounds 9, 10, 15, 16, 18, 33 and 36 showed moderate activity against Gram-negative bacteria. An excellent dose-dependent antibacterial activity was established compared to that of the standard antibiotic ampicillin. Significant anti-biofilm properties were measured quantitatively, showing optical density (O.D) values of 0.51 ± 015, 0.63 ± 0.20, 0.38 ± 0.07 and 0.62 ± 0.11 at 492 nm and the leakage of cellular components by the compounds, such as 17, 20, 25 and 26, increased the O.D. of respective treated samples compared to the control. In addition, the implication of experimental results is discussed in the light of the lack of survivability of planktonic bacteria and biofilm destruction in vitro. These results revealed the great significance of the development of a new generation of synthetic materials with greater efficacy in anti-biofilm properties by targeting to lock the bio-film associated protein Bap in Gram-negative bacteria.

Original languageEnglish
Pages (from-to)5473-5483
Number of pages11
JournalRSC Advances
Volume8
Issue number10
DOIs
Publication statusPublished - 1 Jan 2018
Externally publishedYes

Fingerprint

Biofilms
Bacteria
Density (optical)
Pumps
Antibiotics
Ampicillin
Lipopolysaccharides
Anti-Bacterial Agents
Proteins
Electrons
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Promising bactericidal approach of dihydrazone analogues against bio-film forming Gram-negative bacteria and molecular mechanistic studies. / Rakesh, K. P.; Vivek, H. K.; Manukumar, H. M.; Shantharam, C. S.; Syed Nasir Abbas, Bukhari; Qin, Hua Li; Sridhara, M. B.

In: RSC Advances, Vol. 8, No. 10, 01.01.2018, p. 5473-5483.

Research output: Contribution to journalArticle

Rakesh, K. P. ; Vivek, H. K. ; Manukumar, H. M. ; Shantharam, C. S. ; Syed Nasir Abbas, Bukhari ; Qin, Hua Li ; Sridhara, M. B. / Promising bactericidal approach of dihydrazone analogues against bio-film forming Gram-negative bacteria and molecular mechanistic studies. In: RSC Advances. 2018 ; Vol. 8, No. 10. pp. 5473-5483.
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