Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations

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Abstract

Stem cells that made up of embryonic stem cells and adult stem cells are well known for their ability to self renew and differentiate into matured cells. Adult stem cells isolated from peripheral blood system were used as the study sample. The objective of this study was to determine the relationship between size and proliferation potential of stem cells from 3 different culturing terms, i.e., short (7 days), medium (14 days), and long (30 days) terms. Density gradient centrifugation was applied in isolating mononucleated cells (MNCs) from peripheral blood sample. Isolated MNCs were washed with Hank's Balanced Salt Solution and Phosphate Buffer Saline. The MNCs which cultured in complete media were then subjected to proliferation analysis every day for a time period of 30 days. RT-PCR analyses and cell size measurements were carried out at day 7, 14, and 30. Activation of KIT and SLAMF1 marker genes during RT-PCR analyses indicated that the isolated MNCs were stem cells. Analyses of proliferation rate and cell size showed that short term stem cells have the largest cell size with the lowest proliferation potential. While, medium term stem cells gave rise to a smaller stem cell population with higher proliferation potential compared to short term stem cells. Long term stem cells have smallest cell size with highest proliferation potential. In conclusion, each culturing term cell population had their own sizes and can be isolated based on those sizes.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalMalaysian Applied Biology
Volume42
Issue number2
Publication statusPublished - Dec 2013

Fingerprint

Cell Size
stem cells
Suspensions
Stem Cells
blood
Population
cells
Adult Stem Cells
reverse transcriptase polymerase chain reaction
Polymerase Chain Reaction
Density Gradient Centrifugation
Peripheral Blood Stem Cells
Embryonic Stem Cells
embryonic stem cells
Cultured Cells
Blood Cells
cultured cells
Buffers
Salts
Phosphates

Keywords

  • Proliferation Potential
  • RT-PCR
  • Stem cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

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title = "Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations",
abstract = "Stem cells that made up of embryonic stem cells and adult stem cells are well known for their ability to self renew and differentiate into matured cells. Adult stem cells isolated from peripheral blood system were used as the study sample. The objective of this study was to determine the relationship between size and proliferation potential of stem cells from 3 different culturing terms, i.e., short (7 days), medium (14 days), and long (30 days) terms. Density gradient centrifugation was applied in isolating mononucleated cells (MNCs) from peripheral blood sample. Isolated MNCs were washed with Hank's Balanced Salt Solution and Phosphate Buffer Saline. The MNCs which cultured in complete media were then subjected to proliferation analysis every day for a time period of 30 days. RT-PCR analyses and cell size measurements were carried out at day 7, 14, and 30. Activation of KIT and SLAMF1 marker genes during RT-PCR analyses indicated that the isolated MNCs were stem cells. Analyses of proliferation rate and cell size showed that short term stem cells have the largest cell size with the lowest proliferation potential. While, medium term stem cells gave rise to a smaller stem cell population with higher proliferation potential compared to short term stem cells. Long term stem cells have smallest cell size with highest proliferation potential. In conclusion, each culturing term cell population had their own sizes and can be isolated based on those sizes.",
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author = "Muniandy, {M. K R} and Ruzanna, {A. K.} and {Intan Zarina}, {Z. A.} and {Megat Abdul Wahab}, Rohaya and Sahidan Senafi and {Yeop Majlis}, Burhanuddin and {Zainal Ariffin}, {Shahrul Hisham}",
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T1 - Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations

AU - Muniandy, M. K R

AU - Ruzanna, A. K.

AU - Intan Zarina, Z. A.

AU - Megat Abdul Wahab, Rohaya

AU - Senafi, Sahidan

AU - Yeop Majlis, Burhanuddin

AU - Zainal Ariffin, Shahrul Hisham

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AB - Stem cells that made up of embryonic stem cells and adult stem cells are well known for their ability to self renew and differentiate into matured cells. Adult stem cells isolated from peripheral blood system were used as the study sample. The objective of this study was to determine the relationship between size and proliferation potential of stem cells from 3 different culturing terms, i.e., short (7 days), medium (14 days), and long (30 days) terms. Density gradient centrifugation was applied in isolating mononucleated cells (MNCs) from peripheral blood sample. Isolated MNCs were washed with Hank's Balanced Salt Solution and Phosphate Buffer Saline. The MNCs which cultured in complete media were then subjected to proliferation analysis every day for a time period of 30 days. RT-PCR analyses and cell size measurements were carried out at day 7, 14, and 30. Activation of KIT and SLAMF1 marker genes during RT-PCR analyses indicated that the isolated MNCs were stem cells. Analyses of proliferation rate and cell size showed that short term stem cells have the largest cell size with the lowest proliferation potential. While, medium term stem cells gave rise to a smaller stem cell population with higher proliferation potential compared to short term stem cells. Long term stem cells have smallest cell size with highest proliferation potential. In conclusion, each culturing term cell population had their own sizes and can be isolated based on those sizes.

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