Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces

Ai Wen Tan, Lelia Tay, Chua Kien Hui, Roslina Ahmad, Sheikh Ali Akbar, Belinda Pingguan-Murphy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Two important criteria of an ideal biomaterial in the field of stem cells research are to regulate the cell proliferation without the loss of its pluripotency and to direct the differentiation into a specific cell lineage when desired. The present study describes the influence of TiO2 nanofibrous surface structures on the regulation of proliferation and stemness preservation of adipose-derived stem cells (ADSCs). TiO2 nanofiber arrays were produced in situ onto Ti-6Al-4V substrate via a thermal oxidation process and the successful fabrication of these nanostructures was confirmed by field emission scanning electron microscopy (FESEM), energy dispersive spectrometer (EDS), X-ray diffractometer (XRD), and contact angle measurement. ADSCs were seeded on two types of Ti-6Al-4V surfaces (TiO2 nanofibers and flat control), and their morphology, proliferation, and stemness expression were analyzed using FESEM, AlamarBlue assay, flow cytometry, and quantitative real-time polymerase chain reaction (qRT-PCR) after 2 weeks of incubation, respectively. The results show that ADSCs exhibit better adhesion and significantly enhanced proliferation on the TiO2 nanofibrous surfaces compared to the flat control surfaces. The greater proliferation ability of TiO2 nanofibrous surfaces was further confirmed by the results of cell cycle assay. More importantly, TiO2 nanofibrous surfaces significantly upregulate the expressions of stemness markers Sox-2, Nanog3, Rex-1, and Nestin. These results demonstrate that TiO2 nanofibrous surfaces can be used to enhance cell adhesion and proliferation while simultaneously maintaining the stemness of ADSCs, thereby representing a promising approach for their potential application in the field of bone tissue engineering as well as regenerative therapies.

Original languageEnglish
Pages (from-to)5389-5401
Number of pages13
JournalInternational Journal of Nanomedicine
Volume9
Issue number1
DOIs
Publication statusPublished - 21 Nov 2014

Fingerprint

Stem cells
Stem Cells
Nanofibers
Electron Scanning Microscopy
Cell proliferation
Field emission
Cell Proliferation
Assays
Stem Cell Research
Nestin
Nanostructures
Biocompatible Materials
Cell Lineage
X ray spectrometers
Tissue Engineering
Scanning electron microscopy
Cell Adhesion
Flow cytometry
Control surfaces
Polymerase chain reaction

Keywords

  • Nanofibers
  • Pluripotency
  • Stem cells
  • Thermal oxidation
  • Titania

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

Cite this

Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces. / Tan, Ai Wen; Tay, Lelia; Kien Hui, Chua; Ahmad, Roslina; Akbar, Sheikh Ali; Pingguan-Murphy, Belinda.

In: International Journal of Nanomedicine, Vol. 9, No. 1, 21.11.2014, p. 5389-5401.

Research output: Contribution to journalArticle

Tan, Ai Wen ; Tay, Lelia ; Kien Hui, Chua ; Ahmad, Roslina ; Akbar, Sheikh Ali ; Pingguan-Murphy, Belinda. / Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces. In: International Journal of Nanomedicine. 2014 ; Vol. 9, No. 1. pp. 5389-5401.
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