Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells

Siti Afeefah Mohamad Yusof, Rohaya Megat Abdul Wahab, Sahidan Senafi, Zaidah Zainal Ariffin, Mohamad Abdul Razak, Shahrul Hisham Zainal Ariffin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Stem cell is defined as the ability of the cell to proliferate themselves and differentiate into more than one type of cells. Human mononucleated cell (MN cell) is a suspension cell that was isolated from peripheral blood that was originated from monocyte-machrophage lineage or hematopoietic stem cells. The cells were cultured for 30 days in complete media (CM) which consist of Alpha Minimal Essential Medium (αMEM) with 2% (v/v) Penicillin-Streptomycin and 10% (v/v) Newborn Calf Serum (NBCS). The respective cells were differentiated at day 7 after in vitro proliferation in CM into osteoblastic cells by adding ascorbic acid and β-glycerophosphate. In addition, human recombinant Receptor Activator of Nuclear Factor-β Ligand (hrRANKL) and human recombinant Macrophage-Colony Stimulating Factor (hrM-CSF) were added to induce osteoclastic differentiation of MN cells. Cells that were cultured in CM served as a control and were subjected to the same approach as differentiated cells. The 30 days cultured cells in CM showed a significant increment (p < 0.05) of viable cells compared to day 0 (n=3). The specific activity of Alkaline Phosphatase (ALP) for osteoblast differentiation and Tartrate Resistant Acid Phosphatase (TRAP) for osteoclast differentiation were evaluated via biochemical assay until day 14 and day 10 for osteoblast and osteoclast sample, respectively. ALP and TRAP enzyme showed a significant increment (p< 0.05) after 14 and 10 days of differentiation compared to control cells. As a conclusion, human mononucleated cells are believed to have the potential to be defined as a multipotent stem cell based on their fulfillment of stem cell characteristics.

Original languageEnglish
Pages (from-to)305-309
Number of pages5
JournalSains Malaysiana
Volume40
Issue number4
Publication statusPublished - Apr 2011

Fingerprint

Osteoclasts
Osteoblasts
Cultured Cells
Alkaline Phosphatase
Stem Cells
Multipotent Stem Cells
Glycerophosphates
Macrophage Colony-Stimulating Factor
Streptomycin
Cytoplasmic and Nuclear Receptors
Hematopoietic Stem Cells
Penicillins
Ascorbic Acid
Monocytes
Cell Differentiation
Suspensions
Ligands

Keywords

  • Enzymological analysis
  • Mononucleated
  • Osteoblast
  • Osteoclast

ASJC Scopus subject areas

  • General

Cite this

Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells. / Yusof, Siti Afeefah Mohamad; Megat Abdul Wahab, Rohaya; Senafi, Sahidan; Ariffin, Zaidah Zainal; Razak, Mohamad Abdul; Zainal Ariffin, Shahrul Hisham.

In: Sains Malaysiana, Vol. 40, No. 4, 04.2011, p. 305-309.

Research output: Contribution to journalArticle

Yusof, Siti Afeefah Mohamad ; Megat Abdul Wahab, Rohaya ; Senafi, Sahidan ; Ariffin, Zaidah Zainal ; Razak, Mohamad Abdul ; Zainal Ariffin, Shahrul Hisham. / Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells. In: Sains Malaysiana. 2011 ; Vol. 40, No. 4. pp. 305-309.
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