Production of succinic acid from oil palm empty fruit bunch cellulose using Actinobacillus succinogenes

Satriani Aga Pasma, Rusli Daik, Mohamad Yusof Maskat

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

Succinic acid is a common metabolite in plants, animals and microorganisms. It has been used widely in agricultural, food and pharmaceutical industries. Enzymatic hydrolysate glucose from oil palm empty fruit bunch (OPEFB) cellulose was used as a substrate for succinic acid production using Actinobacillus succinogenes. Using cellulose extraction from OPEFB can enhance the production of glucose as a main substrate for succinic acid production. The highest concentration of glucose produced from enzymatic hydrolysis is 167 mg/mL and the sugar recovery is 0.73 g/g of OPEFB. By optimizing the culture medium for succinic acid fermentation with enzymatic hydrolysate of OPEFB cellulose, the nitrogen sources could be reduced to just only 2.5 g yeast extract and 2.5 g corn step liquor. Batch fermentation was carried out using enzymatic hydrolysate of OPEFB cellulose with yeast extract, corn steep liquor and the salts mixture, 23.5 g/L succinic acid was obtained with consumption of 72 g/L glucose in enzymatic hydrolysate of OPEFB cellulose at 38 hours and 37°C. This study suggests that enzymatic hydrolysate of OPEFB cellulose maybe an alternative substrate for the efficient production of succinic acid by Actinobacillus succinogenes.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages753-759
Number of pages7
Volume1571
DOIs
Publication statusPublished - 2013
Event2013 UKM Faculty of Science and Technology Post-Graduate Colloquium - Selangor
Duration: 3 Jul 20134 Jul 2013

Other

Other2013 UKM Faculty of Science and Technology Post-Graduate Colloquium
CitySelangor
Period3/7/134/7/13

Fingerprint

fruits
cellulose
oils
acids
glucose
corn
fermentation
yeast
culture media
metabolites
microorganisms
sugars
food
animals
hydrolysis
recovery
industries
salts
nitrogen

Keywords

  • Cellulose extraction
  • Enzymatic hydrolysis
  • Fermentation
  • Glucose
  • Renewable sources
  • Succinic acid

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Production of succinic acid from oil palm empty fruit bunch cellulose using Actinobacillus succinogenes. / Pasma, Satriani Aga; Daik, Rusli; Maskat, Mohamad Yusof.

AIP Conference Proceedings. Vol. 1571 2013. p. 753-759.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pasma, SA, Daik, R & Maskat, MY 2013, Production of succinic acid from oil palm empty fruit bunch cellulose using Actinobacillus succinogenes. in AIP Conference Proceedings. vol. 1571, pp. 753-759, 2013 UKM Faculty of Science and Technology Post-Graduate Colloquium, Selangor, 3/7/13. https://doi.org/10.1063/1.4858745
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