Production of high yield sugars from Kappaphycus alvarezii using combined methods of chemical and enzymatic hydrolysis

Faiqah Abd-Rahim, Helmi Wasoh, Mohd Rafein Zakaria, Arbakariya Ariff, Rizal Kapri, Nazaruddin Ramli, Liew Siew-Ling

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    The characteristics and functional properties of polysaccharides extracted from Malaysia's seaweeds have not been fully established to date. Hence, this study was carried out to produce high yield fermentable sugars from Kappaphycus alvarezii for the potential production of biotechnology products such as bioflavours. In order to achieve this objective, the effectiveness of hydrolysis process was studied by using chemical treatments, followed by enzymatic treatments. K.alvarezii were hydrolysed in different heating times and temperatures followed by different types of acids and their concentrations. The optimal conditions for chemical hydrolysis were achieved at 8.0g/100mL of dried powder K.alvarezii in 0.2M H2SO4 and HCl at 110°C for 90min which produced 34.275±0.976g/L and 35.872±3.610g/L, respectively with 42.8% and 44.8% of yield of sugar production. However, there are no significant different between H2SO4 and HCl. Thus, H2SO4 was chose as a catalyst for chemical hydrolysis. As for the combination of chemical and enzymatic hydrolysis, several pH of hydrolysates and incubation temperature were studied. The optimum condition for Celluclast activity was at pH 5.5 and 50°C incubation temperature which produced the highest reducing sugars with an increment of 15.60g/L from the chemical hydrolysis alone. The yield of reducing sugars after combining both methods reached 62.35% (49.92±1.163g/L reducing sugar). From this study, the characterization of these seaweeds can lead to a better understanding of their functional characteristics and promote the exploitation of these natural resources for the production of expensive new biotechnology products.

    Original languageEnglish
    Pages (from-to)309-315
    Number of pages7
    JournalFood Hydrocolloids
    Volume42
    Issue numberP2
    DOIs
    Publication statusPublished - 5 Dec 2014

    Fingerprint

    Kappaphycus alvarezii
    Enzymatic hydrolysis
    enzymatic hydrolysis
    Sugars
    Hydrolysis
    hydrolysis
    reducing sugars
    sugars
    Seaweed
    biotechnology
    functional properties
    macroalgae
    Biotechnology
    Temperature
    temperature
    enzymatic treatment
    chemical treatment
    methodology
    hydrolysates
    catalysts

    Keywords

    • Chemical hydrolysis
    • Enzymatic hydrolysis
    • Reducing sugars
    • Seaweeds

    ASJC Scopus subject areas

    • Food Science
    • Chemical Engineering(all)
    • Chemistry(all)

    Cite this

    Production of high yield sugars from Kappaphycus alvarezii using combined methods of chemical and enzymatic hydrolysis. / Abd-Rahim, Faiqah; Wasoh, Helmi; Zakaria, Mohd Rafein; Ariff, Arbakariya; Kapri, Rizal; Ramli, Nazaruddin; Siew-Ling, Liew.

    In: Food Hydrocolloids, Vol. 42, No. P2, 05.12.2014, p. 309-315.

    Research output: Contribution to journalArticle

    Abd-Rahim, F, Wasoh, H, Zakaria, MR, Ariff, A, Kapri, R, Ramli, N & Siew-Ling, L 2014, 'Production of high yield sugars from Kappaphycus alvarezii using combined methods of chemical and enzymatic hydrolysis', Food Hydrocolloids, vol. 42, no. P2, pp. 309-315. https://doi.org/10.1016/j.foodhyd.2014.05.017
    Abd-Rahim, Faiqah ; Wasoh, Helmi ; Zakaria, Mohd Rafein ; Ariff, Arbakariya ; Kapri, Rizal ; Ramli, Nazaruddin ; Siew-Ling, Liew. / Production of high yield sugars from Kappaphycus alvarezii using combined methods of chemical and enzymatic hydrolysis. In: Food Hydrocolloids. 2014 ; Vol. 42, No. P2. pp. 309-315.
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    AU - Wasoh, Helmi

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    AU - Ariff, Arbakariya

    AU - Kapri, Rizal

    AU - Ramli, Nazaruddin

    AU - Siew-Ling, Liew

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