Production of fused protein consisting of Vp2 from infectious bursal disease virus and hn from newcastle disease virus in Escherichia coli

N. A.A.N. Muhammad, Raha Ahmad-Raus, N. M. Amin, A. S. Syamsiah, Farah Diba Abu Bakar, M. S. Ghazali

Research output: Contribution to journalArticle

Abstract

Viral protein 2 (VP2) of infectious bursal disease virus (IBDV) and hemagglutinin-neuraminidase (HN) of Newcastle disease virus (NDV) are viral surface proteins which contain epitopes that are able to induce neutralizing antibodies for protection against Newcastle disease and infectious bursal disease. In the present study, recombinant fused protein consisting of VP2 and partial HN protein was produced. The fused protein was made by fusing VP2 gene to full length (flHN) and partial HN (pHN) gene, separately and cloned it into pRSETB expression vector. The recombinant construct is then transformed into E. coli BL21 (DE3) for production of the fused protein. The fused VP2-pHN protein was successfully produced in E. coli BL21 (DE3) strain with the size of 75 kDa detected by anti-His monoclonal antibody via Western blot analysis.

Original languageEnglish
Pages (from-to)47-55
Number of pages9
JournalAsia-Pacific Journal of Molecular Biology and Biotechnology
Volume25
Issue number1
Publication statusPublished - 1 Jun 2017

Fingerprint

Infectious bursal disease virus
Newcastle disease virus
Viral Proteins
Escherichia coli
Hemagglutinins
Neuraminidase
Proteins
HN Protein
Newcastle Disease
Neutralizing Antibodies
Recombinant Proteins
Communicable Diseases
Epitopes
Membrane Proteins
Western Blotting
Monoclonal Antibodies
Genes

Keywords

  • Escherichia coli
  • Fused protein
  • Hemagglutinin-neuraminidase
  • Infectious bursal disease
  • Newcastle disease
  • Viral protein 2

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

Production of fused protein consisting of Vp2 from infectious bursal disease virus and hn from newcastle disease virus in Escherichia coli. / Muhammad, N. A.A.N.; Ahmad-Raus, Raha; Amin, N. M.; Syamsiah, A. S.; Abu Bakar, Farah Diba; Ghazali, M. S.

In: Asia-Pacific Journal of Molecular Biology and Biotechnology, Vol. 25, No. 1, 01.06.2017, p. 47-55.

Research output: Contribution to journalArticle

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