Production of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1

Optimization of carbon and nitrogen concentration in the feed medium using central composite design

Wan Salwanis Wan Md Zain, Rosli Md Illias, Madihah Md Salleh, Osman Hassan, Roshanida A. Rahman, Aidil Abdul Hamid

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Optimisation of nutrient feeding was developed to overcome the limitation in batch fermentation and to increase the CGTase production from Bacillus sp. TS1-1 in fed batch fermentation. Optimisation of the C/N ratio in the feed stream was conducted in a 5 l fermenter, where feeding was initiated at constant rate of 0.02 h-1. In our initial screening process, the addition of nitrogen source boosted the growth of the microbes, but on the other hand reduced the CGTase production. The amount of tapioca starch and yeast extract was optimised in order to obtain a sufficient growth and thus, increased the CGTase production. Results were analysed using three-dimensional response surface plot, and the optimised values of carbon and nitrogen concentration of 3.30% (w/v) and 0.13% (w/v) were obtained, respectively. CGTase activity increased up to 80.12 U/ml, which is 13.94% higher as compared to batch fermentation (70.32 U/ml). This also led to 14.54% increment of CGTase production in fed batch culture as compared to the production before the optimisation. The CGTase activity obtained was close to the predicted value, which is 78.05 U/ml.

Original languageEnglish
Pages (from-to)26-33
Number of pages8
JournalBiochemical Engineering Journal
Volume33
Issue number1
DOIs
Publication statusPublished - Jan 2007

Fingerprint

Cyclodextrins
Bacilli
Bacillus
Fermentation
Nitrogen
Carbon
Composite materials
Manihot
Batch Cell Culture Techniques
Growth
Starch
Fermenters
Yeasts
Food
Yeast
Nutrients
Screening
cyclomaltodextrin glucanotransferase

Keywords

  • C/N ratio
  • Central composite design
  • Cyclodextrin glucanotransferase
  • Fed batch

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Chemical Engineering(all)

Cite this

Production of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1 : Optimization of carbon and nitrogen concentration in the feed medium using central composite design. / Zain, Wan Salwanis Wan Md; Illias, Rosli Md; Salleh, Madihah Md; Hassan, Osman; Rahman, Roshanida A.; Abdul Hamid, Aidil.

In: Biochemical Engineering Journal, Vol. 33, No. 1, 01.2007, p. 26-33.

Research output: Contribution to journalArticle

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