Production of cyclodextrin glucanotransferase (CGTase) from alkalophilic Bacillus sp. TS1-1

Media optimization using experimental design

Mohd Khairizal Mahat, Rosli Md Illias, Roshanida A. Rahman, Noor Aini Abd Rashid, Nik Azmi Nik Mahmood, Osman Hassan, Suraini Abdul Aziz, Kamarulzaman Kamaruddin

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Cyclodextrin glucanotransferase (CGTase) was produced when the Bacillus sp. TS1-1 was grown in a medium containing sago starch, yeast extract, phosphorus and mineral salt sources, using shake flask mode at 37°C for 24 h. Response surface methodology (RSM) was applied to optimize the medium constituents with respect to CGTase production and activity. A 2 4 full factorial design (first order model) was carried out to identify the significant effect of medium components towards CGTase production. The variables involved in this initial screening study were sago starch, yeast extract, K 2HPO 4 and MgSO 4·7H 2O. Statistical analysis of results have shown that only sago starch and yeast extract have a significant effect on CGTase production. A second-order model was proposed by using 2 2 central composite design to represent the production CGTase activity as a function of sago starch and yeast extract. The optimized values of 1.48% and 1.89% of sago starch and yeast extract was obtained, respectively. Under these proposed optimized conditions, the model predicted a CGTase activity of 79.66 U/ml and via experimental rechecking the model, an activity of 84 U/ml was attained.

Original languageEnglish
Pages (from-to)467-473
Number of pages7
JournalEnzyme and Microbial Technology
Volume35
Issue number5
DOIs
Publication statusPublished - 6 Oct 2004

Fingerprint

Cyclodextrins
Bacilli
Design of experiments
Bacillus
Starch
Research Design
Yeast
Yeasts
Phosphorus
Minerals
cyclomaltodextrin glucanotransferase
Statistical methods
Screening
Theoretical Models
Salts
Composite materials

Keywords

  • Cyclodextrin
  • Cyclodextrin glucanotransferase
  • Medium optimization
  • Response surface methodology

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Production of cyclodextrin glucanotransferase (CGTase) from alkalophilic Bacillus sp. TS1-1 : Media optimization using experimental design. / Mahat, Mohd Khairizal; Illias, Rosli Md; Rahman, Roshanida A.; Rashid, Noor Aini Abd; Mahmood, Nik Azmi Nik; Hassan, Osman; Aziz, Suraini Abdul; Kamaruddin, Kamarulzaman.

In: Enzyme and Microbial Technology, Vol. 35, No. 5, 06.10.2004, p. 467-473.

Research output: Contribution to journalArticle

Mahat, Mohd Khairizal ; Illias, Rosli Md ; Rahman, Roshanida A. ; Rashid, Noor Aini Abd ; Mahmood, Nik Azmi Nik ; Hassan, Osman ; Aziz, Suraini Abdul ; Kamaruddin, Kamarulzaman. / Production of cyclodextrin glucanotransferase (CGTase) from alkalophilic Bacillus sp. TS1-1 : Media optimization using experimental design. In: Enzyme and Microbial Technology. 2004 ; Vol. 35, No. 5. pp. 467-473.
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