Abstract
Butanol was produced from pretreated deoiled rice bran (DRB) in a batch culture of Clostridium acetobutylicum YM1. The DRB was pretreated by acetyl chloride to produce fermentable sugars prior to butanol fermentation. Pretreatment of DRB using 1% acetyl chloride (AC-DRB) resulted in sufficient fermentable sugars (30.88 g/L), which was comparable to that produced by using 1% sulfuric acid (33.5 g/L). Pretreated AC-DRB contained 18.08 g/L glucose, 9.95 g/L xylose, and 2.86 g/L cellobiose. Detoxification of AC-DRB was performed to remove the fermentation inhibitors, such as furfural, 5-hydroxymethyl furfural (HMF), acetic acid, formic acid, and levulinic acid with the removal efficiencies of 92.98%, 98.82%, 51.53%, 38.72%, and 96.21%, respectively, using charcoal. The detoxification with charcoal was more efficient compared to that with XAD-4 resin. Acetone-butanol-ethanol (ABE) fermentation of detoxified AC-DRB (with 1% AC) by XAD-4 produced 5.64 g/L butanol, while detoxification with charcoal of AC-DRB (with 1% AC) produced 6.48 g/L butanol. In detoxified AC-DRB with charcoal, the maximum butanol and ABE yield of 6.48 g/L and 11.82 g/L, respectively, were achieved. This study is the first reported treatment of biomass using acetyl chloride, which was used as a pretreatment method for successful butanol production.
| Original language | English |
|---|---|
| Pages (from-to) | 8505-8518 |
| Number of pages | 14 |
| Journal | BioResources |
| Volume | 12 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Nov 2017 |
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Keywords
- Acetyl chloride Pretreatment
- Butanol
- Clostridium acetobutylicum YM1
- Deoiled rice bran
ASJC Scopus subject areas
- Environmental Engineering
- Bioengineering
- Waste Management and Disposal
Cite this
Production of butanol from acetyl chloride-treated Deoiled rice bran by Clostridium acetobutylicum YM1. / Al-Shorgani, Najeeb Kaid Nasser; Al-Tabib, Abdualati Ibrahim; Kalil, Mohd. Sahaid.
In: BioResources, Vol. 12, No. 4, 01.11.2017, p. 8505-8518.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of butanol from acetyl chloride-treated Deoiled rice bran by Clostridium acetobutylicum YM1
AU - Al-Shorgani, Najeeb Kaid Nasser
AU - Al-Tabib, Abdualati Ibrahim
AU - Kalil, Mohd. Sahaid
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Butanol was produced from pretreated deoiled rice bran (DRB) in a batch culture of Clostridium acetobutylicum YM1. The DRB was pretreated by acetyl chloride to produce fermentable sugars prior to butanol fermentation. Pretreatment of DRB using 1% acetyl chloride (AC-DRB) resulted in sufficient fermentable sugars (30.88 g/L), which was comparable to that produced by using 1% sulfuric acid (33.5 g/L). Pretreated AC-DRB contained 18.08 g/L glucose, 9.95 g/L xylose, and 2.86 g/L cellobiose. Detoxification of AC-DRB was performed to remove the fermentation inhibitors, such as furfural, 5-hydroxymethyl furfural (HMF), acetic acid, formic acid, and levulinic acid with the removal efficiencies of 92.98%, 98.82%, 51.53%, 38.72%, and 96.21%, respectively, using charcoal. The detoxification with charcoal was more efficient compared to that with XAD-4 resin. Acetone-butanol-ethanol (ABE) fermentation of detoxified AC-DRB (with 1% AC) by XAD-4 produced 5.64 g/L butanol, while detoxification with charcoal of AC-DRB (with 1% AC) produced 6.48 g/L butanol. In detoxified AC-DRB with charcoal, the maximum butanol and ABE yield of 6.48 g/L and 11.82 g/L, respectively, were achieved. This study is the first reported treatment of biomass using acetyl chloride, which was used as a pretreatment method for successful butanol production.
AB - Butanol was produced from pretreated deoiled rice bran (DRB) in a batch culture of Clostridium acetobutylicum YM1. The DRB was pretreated by acetyl chloride to produce fermentable sugars prior to butanol fermentation. Pretreatment of DRB using 1% acetyl chloride (AC-DRB) resulted in sufficient fermentable sugars (30.88 g/L), which was comparable to that produced by using 1% sulfuric acid (33.5 g/L). Pretreated AC-DRB contained 18.08 g/L glucose, 9.95 g/L xylose, and 2.86 g/L cellobiose. Detoxification of AC-DRB was performed to remove the fermentation inhibitors, such as furfural, 5-hydroxymethyl furfural (HMF), acetic acid, formic acid, and levulinic acid with the removal efficiencies of 92.98%, 98.82%, 51.53%, 38.72%, and 96.21%, respectively, using charcoal. The detoxification with charcoal was more efficient compared to that with XAD-4 resin. Acetone-butanol-ethanol (ABE) fermentation of detoxified AC-DRB (with 1% AC) by XAD-4 produced 5.64 g/L butanol, while detoxification with charcoal of AC-DRB (with 1% AC) produced 6.48 g/L butanol. In detoxified AC-DRB with charcoal, the maximum butanol and ABE yield of 6.48 g/L and 11.82 g/L, respectively, were achieved. This study is the first reported treatment of biomass using acetyl chloride, which was used as a pretreatment method for successful butanol production.
KW - Acetyl chloride Pretreatment
KW - Butanol
KW - Clostridium acetobutylicum YM1
KW - Deoiled rice bran
UR - http://www.scopus.com/inward/record.url?scp=85032674023&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85032674023&partnerID=8YFLogxK
U2 - 10.15376/biores.12.4.8505-8518
DO - 10.15376/biores.12.4.8505-8518
M3 - Article
VL - 12
SP - 8505
EP - 8518
JO - BioResources
JF - BioResources
SN - 1930-2126
IS - 4
ER -