Production of butanol from acetyl chloride-treated Deoiled rice bran by Clostridium acetobutylicum YM1

Najeeb Kaid Nasser Al-Shorgani, Abdualati Ibrahim Al-Tabib, Mohd. Sahaid Kalil

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Butanol was produced from pretreated deoiled rice bran (DRB) in a batch culture of Clostridium acetobutylicum YM1. The DRB was pretreated by acetyl chloride to produce fermentable sugars prior to butanol fermentation. Pretreatment of DRB using 1% acetyl chloride (AC-DRB) resulted in sufficient fermentable sugars (30.88 g/L), which was comparable to that produced by using 1% sulfuric acid (33.5 g/L). Pretreated AC-DRB contained 18.08 g/L glucose, 9.95 g/L xylose, and 2.86 g/L cellobiose. Detoxification of AC-DRB was performed to remove the fermentation inhibitors, such as furfural, 5-hydroxymethyl furfural (HMF), acetic acid, formic acid, and levulinic acid with the removal efficiencies of 92.98%, 98.82%, 51.53%, 38.72%, and 96.21%, respectively, using charcoal. The detoxification with charcoal was more efficient compared to that with XAD-4 resin. Acetone-butanol-ethanol (ABE) fermentation of detoxified AC-DRB (with 1% AC) by XAD-4 produced 5.64 g/L butanol, while detoxification with charcoal of AC-DRB (with 1% AC) produced 6.48 g/L butanol. In detoxified AC-DRB with charcoal, the maximum butanol and ABE yield of 6.48 g/L and 11.82 g/L, respectively, were achieved. This study is the first reported treatment of biomass using acetyl chloride, which was used as a pretreatment method for successful butanol production.

Original languageEnglish
Pages (from-to)8505-8518
Number of pages14
JournalBioResources
Volume12
Issue number4
DOIs
Publication statusPublished - 1 Nov 2017

Fingerprint

Clostridium
Butanols
Butenes
rice
chloride
Charcoal
Detoxification
charcoal
Fermentation
detoxification
Furfural
formic acid
fermentation
Acetone
Sugars
acetone
Ethanol
ethanol
sugar
Cellobiose

Keywords

  • Acetyl chloride Pretreatment
  • Butanol
  • Clostridium acetobutylicum YM1
  • Deoiled rice bran

ASJC Scopus subject areas

  • Environmental Engineering
  • Bioengineering
  • Waste Management and Disposal

Cite this

Production of butanol from acetyl chloride-treated Deoiled rice bran by Clostridium acetobutylicum YM1. / Al-Shorgani, Najeeb Kaid Nasser; Al-Tabib, Abdualati Ibrahim; Kalil, Mohd. Sahaid.

In: BioResources, Vol. 12, No. 4, 01.11.2017, p. 8505-8518.

Research output: Contribution to journalArticle

Al-Shorgani, Najeeb Kaid Nasser ; Al-Tabib, Abdualati Ibrahim ; Kalil, Mohd. Sahaid. / Production of butanol from acetyl chloride-treated Deoiled rice bran by Clostridium acetobutylicum YM1. In: BioResources. 2017 ; Vol. 12, No. 4. pp. 8505-8518.
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abstract = "Butanol was produced from pretreated deoiled rice bran (DRB) in a batch culture of Clostridium acetobutylicum YM1. The DRB was pretreated by acetyl chloride to produce fermentable sugars prior to butanol fermentation. Pretreatment of DRB using 1{\%} acetyl chloride (AC-DRB) resulted in sufficient fermentable sugars (30.88 g/L), which was comparable to that produced by using 1{\%} sulfuric acid (33.5 g/L). Pretreated AC-DRB contained 18.08 g/L glucose, 9.95 g/L xylose, and 2.86 g/L cellobiose. Detoxification of AC-DRB was performed to remove the fermentation inhibitors, such as furfural, 5-hydroxymethyl furfural (HMF), acetic acid, formic acid, and levulinic acid with the removal efficiencies of 92.98{\%}, 98.82{\%}, 51.53{\%}, 38.72{\%}, and 96.21{\%}, respectively, using charcoal. The detoxification with charcoal was more efficient compared to that with XAD-4 resin. Acetone-butanol-ethanol (ABE) fermentation of detoxified AC-DRB (with 1{\%} AC) by XAD-4 produced 5.64 g/L butanol, while detoxification with charcoal of AC-DRB (with 1{\%} AC) produced 6.48 g/L butanol. In detoxified AC-DRB with charcoal, the maximum butanol and ABE yield of 6.48 g/L and 11.82 g/L, respectively, were achieved. This study is the first reported treatment of biomass using acetyl chloride, which was used as a pretreatment method for successful butanol production.",
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AB - Butanol was produced from pretreated deoiled rice bran (DRB) in a batch culture of Clostridium acetobutylicum YM1. The DRB was pretreated by acetyl chloride to produce fermentable sugars prior to butanol fermentation. Pretreatment of DRB using 1% acetyl chloride (AC-DRB) resulted in sufficient fermentable sugars (30.88 g/L), which was comparable to that produced by using 1% sulfuric acid (33.5 g/L). Pretreated AC-DRB contained 18.08 g/L glucose, 9.95 g/L xylose, and 2.86 g/L cellobiose. Detoxification of AC-DRB was performed to remove the fermentation inhibitors, such as furfural, 5-hydroxymethyl furfural (HMF), acetic acid, formic acid, and levulinic acid with the removal efficiencies of 92.98%, 98.82%, 51.53%, 38.72%, and 96.21%, respectively, using charcoal. The detoxification with charcoal was more efficient compared to that with XAD-4 resin. Acetone-butanol-ethanol (ABE) fermentation of detoxified AC-DRB (with 1% AC) by XAD-4 produced 5.64 g/L butanol, while detoxification with charcoal of AC-DRB (with 1% AC) produced 6.48 g/L butanol. In detoxified AC-DRB with charcoal, the maximum butanol and ABE yield of 6.48 g/L and 11.82 g/L, respectively, were achieved. This study is the first reported treatment of biomass using acetyl chloride, which was used as a pretreatment method for successful butanol production.

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