Production of butanol by Clostridium saccharoperbutylacetonicum N1-4 from palm kernel cake in acetone-butanol-ethanol fermentation using an empirical model

Hafiza Shukor, Najeeb Kaid Nasser Al-Shorgani, Peyman Abdeshahian, Aidil Abdul Hamid, Nurina Anuar, Norliza Abd Rahman, Mohd. Sahaid Kalil

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Abstract

Palm kernel cake (PKC) was used for biobutanol production by Clostridium saccharoperbutylacetonicum N1-4 in acetone-butanol-ethanol (ABE) fermentation. PKC was subjected to acid hydrolysis pretreatment and hydrolysates released were detoxified by XAD-4 resin. The effect of pH, temperature and inoculum size on butanol production was evaluated using an empirical model. Twenty ABE fermentations were run according to an experimental design. Experimental results revealed that XAD-4 resin removed 50% furfural and 77.42% hydroxymethyl furfural. The analysis of the empirical model showed that linear effect of inoculums size with quadratic effect of pH and inoculum size influenced butanol production at 99% probability level (P<. 0.01). The optimum conditions for butanol production were pH 6.28, temperature of 28. °C and inoculum size of 15.9%. ABE fermentation was carried out under optimum conditions which 0.1. g/L butanol was obtained. Butanol production was enhanced by diluting PKC hydrolysate up to 70% in which 3.59. g/L butanol was produced.

Original languageEnglish
Pages (from-to)565-573
Number of pages9
JournalBioresource Technology
Volume170
DOIs
Publication statusPublished - 2014

Fingerprint

Clostridium
Butanols
Acetone
acetone
Butenes
Fermentation
fermentation
ethanol
Ethanol
resin
Furaldehyde
Furfural
experimental design
hydrolysis
temperature
Resins
acid
effect
Design of experiments
Hydrolysis

Keywords

  • Acid pretreatment
  • Butanol production
  • Clostridium saccharoperbutylacetonicum N1-4
  • Empirical model
  • Palm kernel cake

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

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title = "Production of butanol by Clostridium saccharoperbutylacetonicum N1-4 from palm kernel cake in acetone-butanol-ethanol fermentation using an empirical model",
abstract = "Palm kernel cake (PKC) was used for biobutanol production by Clostridium saccharoperbutylacetonicum N1-4 in acetone-butanol-ethanol (ABE) fermentation. PKC was subjected to acid hydrolysis pretreatment and hydrolysates released were detoxified by XAD-4 resin. The effect of pH, temperature and inoculum size on butanol production was evaluated using an empirical model. Twenty ABE fermentations were run according to an experimental design. Experimental results revealed that XAD-4 resin removed 50{\%} furfural and 77.42{\%} hydroxymethyl furfural. The analysis of the empirical model showed that linear effect of inoculums size with quadratic effect of pH and inoculum size influenced butanol production at 99{\%} probability level (P<. 0.01). The optimum conditions for butanol production were pH 6.28, temperature of 28. °C and inoculum size of 15.9{\%}. ABE fermentation was carried out under optimum conditions which 0.1. g/L butanol was obtained. Butanol production was enhanced by diluting PKC hydrolysate up to 70{\%} in which 3.59. g/L butanol was produced.",
keywords = "Acid pretreatment, Butanol production, Clostridium saccharoperbutylacetonicum N1-4, Empirical model, Palm kernel cake",
author = "Hafiza Shukor and Al-Shorgani, {Najeeb Kaid Nasser} and Peyman Abdeshahian and {Abdul Hamid}, Aidil and Nurina Anuar and {Abd Rahman}, Norliza and Kalil, {Mohd. Sahaid}",
year = "2014",
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pages = "565--573",
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issn = "0960-8524",
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T1 - Production of butanol by Clostridium saccharoperbutylacetonicum N1-4 from palm kernel cake in acetone-butanol-ethanol fermentation using an empirical model

AU - Shukor, Hafiza

AU - Al-Shorgani, Najeeb Kaid Nasser

AU - Abdeshahian, Peyman

AU - Abdul Hamid, Aidil

AU - Anuar, Nurina

AU - Abd Rahman, Norliza

AU - Kalil, Mohd. Sahaid

PY - 2014

Y1 - 2014

N2 - Palm kernel cake (PKC) was used for biobutanol production by Clostridium saccharoperbutylacetonicum N1-4 in acetone-butanol-ethanol (ABE) fermentation. PKC was subjected to acid hydrolysis pretreatment and hydrolysates released were detoxified by XAD-4 resin. The effect of pH, temperature and inoculum size on butanol production was evaluated using an empirical model. Twenty ABE fermentations were run according to an experimental design. Experimental results revealed that XAD-4 resin removed 50% furfural and 77.42% hydroxymethyl furfural. The analysis of the empirical model showed that linear effect of inoculums size with quadratic effect of pH and inoculum size influenced butanol production at 99% probability level (P<. 0.01). The optimum conditions for butanol production were pH 6.28, temperature of 28. °C and inoculum size of 15.9%. ABE fermentation was carried out under optimum conditions which 0.1. g/L butanol was obtained. Butanol production was enhanced by diluting PKC hydrolysate up to 70% in which 3.59. g/L butanol was produced.

AB - Palm kernel cake (PKC) was used for biobutanol production by Clostridium saccharoperbutylacetonicum N1-4 in acetone-butanol-ethanol (ABE) fermentation. PKC was subjected to acid hydrolysis pretreatment and hydrolysates released were detoxified by XAD-4 resin. The effect of pH, temperature and inoculum size on butanol production was evaluated using an empirical model. Twenty ABE fermentations were run according to an experimental design. Experimental results revealed that XAD-4 resin removed 50% furfural and 77.42% hydroxymethyl furfural. The analysis of the empirical model showed that linear effect of inoculums size with quadratic effect of pH and inoculum size influenced butanol production at 99% probability level (P<. 0.01). The optimum conditions for butanol production were pH 6.28, temperature of 28. °C and inoculum size of 15.9%. ABE fermentation was carried out under optimum conditions which 0.1. g/L butanol was obtained. Butanol production was enhanced by diluting PKC hydrolysate up to 70% in which 3.59. g/L butanol was produced.

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KW - Empirical model

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