Production of bioactive peptides using enzymatic hydrolysis and identification antioxidative peptides from patin (Pangasius sutchi) sarcoplasmic protein hydolysate

Leila Najafian, Abd. Salam Babji

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Patin sarcoplasmic protein was hydrolysed with Alcalase and papain. The antioxidant activities of sarcoplasmic protein hydrolysates (SPHs) were assessed by evaluating 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical-scavenging activities, reducing power and metal chelating activity. The Alcalase hydrolysate was purified by ultrafiltration, ion exchange and gel filtration chromatography and RP-HPLC. The most potent fraction (SI 3) obtained from RP-HPLC showed DPPH radical-scavenging activity by 1.83 times higher than SPH. Three antioxidant peptide sequences, Asp-Pro-Gln-His-Pro-Val-Met-Pro-Arg, Leu-Val-Val-Asp-Ile-Pro-Ala-Ala-Leu-Gln-His-Ala and Gly-Val-Asp-Asn-Pro-Gly-His-Pro, were identified by HPLC connected to electrospray ionisation-time-of-flight mass spectrometer (ESI-TOF MS/MS). The presence of a hydrophobic amino acid (Val), hydrophilic amino acids (His and Pro) and an acidic amino acid (Asp) in the peptide sequences is believed to contribute to high antioxidant activity of SPH. Hence, antioxidative peptides from patin protein may be produced for development as natural antioxidant ingredients in functional foods.

Original languageEnglish
Pages (from-to)280-289
Number of pages10
JournalJournal of Functional Foods
Volume9
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Pangasianodon hypophthalmus
protein hydrolysates
enzymatic hydrolysis
Protein Hydrolysates
Hydrolysis
subtilisin
high performance liquid chromatography
Antioxidants
peptides
Subtilisins
Peptides
amino acids
High Pressure Liquid Chromatography
antioxidant activity
antioxidants
Proteins
proteins
papain
sulfonic acid
ion exchange

Keywords

  • Amino acid sequencing
  • Antioxidative peptides
  • Enzymatic hydrolysis
  • Patin (Pangasius sutchi)
  • Sarcoplasmic fractions

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Production of bioactive peptides using enzymatic hydrolysis and identification antioxidative peptides from patin (Pangasius sutchi) sarcoplasmic protein hydolysate. / Najafian, Leila; Babji, Abd. Salam.

In: Journal of Functional Foods, Vol. 9, No. 1, 2014, p. 280-289.

Research output: Contribution to journalArticle

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