Production of Acetone, Butanol and Ethanol (ABE) by Clostridium saccharoperbutylacetonicum N1-4 with different immobilization systems

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The objective of this study is to investigate the production of Acetone, Butanol and Ethanol (ABE) by immobilized cells of C. saccharoperbutylacetonicum N1-4 (ATCC 13564) under anaerobic batch culture system. Two different methods of immobilization, active immobilization in alginate and passive immobilization by employing stainless steel scrubber, nylon scrubber, polyurethane with uniform pore's size, polyurethane with different pore's size and palm oil empty fruit bunch fiber were studied. Immobilization in alginate was carried out on the effect of cell's age, initial culture pH and temperature on the production of ABE. Immobilized solventogenic cells (18 h) produced the highest total solvents concentration compared to the other phases with productivity of 0.325 g L -1 h-1. The highest solvents production by active immobilization of cells was obtained at pH 6.0 with 30°C with productivity of 0.336 g L-1 h-1. Polyurethane with different pore's size is significantly better than other materials tested with production of solvents productivity and YP/S at 3.2 times and 1.9 times, respectively compared to free cells after 24 h fermentation. We conclude that passive immobilization technique increases the productivity (215.12 %) and YP/S (88.37 %) of solvents by C. saccharoperbutylacetonicum N1-4.

Original languageEnglish
Pages (from-to)1923-1928
Number of pages6
JournalPakistan Journal of Biological Sciences
Volume9
Issue number10
DOIs
Publication statusPublished - Oct 2006

Fingerprint

Clostridium saccharoperbutylacetonicum
butanol
acetone
polyurethanes
ethanol
scrubbers
immobilized cells
alginates
palm oils
stainless steel
cells
nylon
fermentation
fruits
methodology
temperature

Keywords

  • Acetone-butanol-ethanol (ABE)
  • Batch culture
  • C. saccharoperbutylacetonicum N1-4
  • Passive and active immobilization

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

@article{5148dd9a67e14733a6aeecfae5525ffe,
title = "Production of Acetone, Butanol and Ethanol (ABE) by Clostridium saccharoperbutylacetonicum N1-4 with different immobilization systems",
abstract = "The objective of this study is to investigate the production of Acetone, Butanol and Ethanol (ABE) by immobilized cells of C. saccharoperbutylacetonicum N1-4 (ATCC 13564) under anaerobic batch culture system. Two different methods of immobilization, active immobilization in alginate and passive immobilization by employing stainless steel scrubber, nylon scrubber, polyurethane with uniform pore's size, polyurethane with different pore's size and palm oil empty fruit bunch fiber were studied. Immobilization in alginate was carried out on the effect of cell's age, initial culture pH and temperature on the production of ABE. Immobilized solventogenic cells (18 h) produced the highest total solvents concentration compared to the other phases with productivity of 0.325 g L -1 h-1. The highest solvents production by active immobilization of cells was obtained at pH 6.0 with 30°C with productivity of 0.336 g L-1 h-1. Polyurethane with different pore's size is significantly better than other materials tested with production of solvents productivity and YP/S at 3.2 times and 1.9 times, respectively compared to free cells after 24 h fermentation. We conclude that passive immobilization technique increases the productivity (215.12 {\%}) and YP/S (88.37 {\%}) of solvents by C. saccharoperbutylacetonicum N1-4.",
keywords = "Acetone-butanol-ethanol (ABE), Batch culture, C. saccharoperbutylacetonicum N1-4, Passive and active immobilization",
author = "Saleha Shamsudin and Kalil, {Mohd. Sahaid} and {Wan Yusoff}, {Wan Mohtar}",
year = "2006",
month = "10",
doi = "10.3923/pjbs.2006.1923.1928",
language = "English",
volume = "9",
pages = "1923--1928",
journal = "Pakistan Journal of Biological Sciences",
issn = "1028-8880",
publisher = "Asian Network for Scientific Information",
number = "10",

}

TY - JOUR

T1 - Production of Acetone, Butanol and Ethanol (ABE) by Clostridium saccharoperbutylacetonicum N1-4 with different immobilization systems

AU - Shamsudin, Saleha

AU - Kalil, Mohd. Sahaid

AU - Wan Yusoff, Wan Mohtar

PY - 2006/10

Y1 - 2006/10

N2 - The objective of this study is to investigate the production of Acetone, Butanol and Ethanol (ABE) by immobilized cells of C. saccharoperbutylacetonicum N1-4 (ATCC 13564) under anaerobic batch culture system. Two different methods of immobilization, active immobilization in alginate and passive immobilization by employing stainless steel scrubber, nylon scrubber, polyurethane with uniform pore's size, polyurethane with different pore's size and palm oil empty fruit bunch fiber were studied. Immobilization in alginate was carried out on the effect of cell's age, initial culture pH and temperature on the production of ABE. Immobilized solventogenic cells (18 h) produced the highest total solvents concentration compared to the other phases with productivity of 0.325 g L -1 h-1. The highest solvents production by active immobilization of cells was obtained at pH 6.0 with 30°C with productivity of 0.336 g L-1 h-1. Polyurethane with different pore's size is significantly better than other materials tested with production of solvents productivity and YP/S at 3.2 times and 1.9 times, respectively compared to free cells after 24 h fermentation. We conclude that passive immobilization technique increases the productivity (215.12 %) and YP/S (88.37 %) of solvents by C. saccharoperbutylacetonicum N1-4.

AB - The objective of this study is to investigate the production of Acetone, Butanol and Ethanol (ABE) by immobilized cells of C. saccharoperbutylacetonicum N1-4 (ATCC 13564) under anaerobic batch culture system. Two different methods of immobilization, active immobilization in alginate and passive immobilization by employing stainless steel scrubber, nylon scrubber, polyurethane with uniform pore's size, polyurethane with different pore's size and palm oil empty fruit bunch fiber were studied. Immobilization in alginate was carried out on the effect of cell's age, initial culture pH and temperature on the production of ABE. Immobilized solventogenic cells (18 h) produced the highest total solvents concentration compared to the other phases with productivity of 0.325 g L -1 h-1. The highest solvents production by active immobilization of cells was obtained at pH 6.0 with 30°C with productivity of 0.336 g L-1 h-1. Polyurethane with different pore's size is significantly better than other materials tested with production of solvents productivity and YP/S at 3.2 times and 1.9 times, respectively compared to free cells after 24 h fermentation. We conclude that passive immobilization technique increases the productivity (215.12 %) and YP/S (88.37 %) of solvents by C. saccharoperbutylacetonicum N1-4.

KW - Acetone-butanol-ethanol (ABE)

KW - Batch culture

KW - C. saccharoperbutylacetonicum N1-4

KW - Passive and active immobilization

UR - http://www.scopus.com/inward/record.url?scp=33750007381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750007381&partnerID=8YFLogxK

U2 - 10.3923/pjbs.2006.1923.1928

DO - 10.3923/pjbs.2006.1923.1928

M3 - Article

VL - 9

SP - 1923

EP - 1928

JO - Pakistan Journal of Biological Sciences

JF - Pakistan Journal of Biological Sciences

SN - 1028-8880

IS - 10

ER -