Production of Acetone, Butanol, and Ethanol (ABE) by clostridium acetobutylicum YM1 from pretreated palm kernel cake in batch culture fermentation

Abdualati Ibrahim Al-Tabib, Najeeb Kaid Nasser Al-Shorgani, Hassimi Abu Hasan, Aidil Abdul Hamid, Mohd. Sahaid Kalil

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The viability of most fermentation processes is very much dependent on the cheap fermentation medium used. Palm kernel cake (PKC) is an abundant biomass generated from the palm oil processing industry that can be used as the carbon source for the growth and production of acetone-butanol-ethanol fermentation (ABE) by Clostridia. In this study, ABE production from the fermentation of PKC using Clostridium acetobutylicum YM1 in a batch culture was conducted. The PKC was subjected to treatment with acids (sulphuric and hydrochloric acids), alkali (sodium hydroxide and alkaline peroxide), enzymatic hydrolysis, and hydrothermal treatment (in autoclave). The sulphuric acid-treated PKC (2% SAPKC) method produced the highest concentration of reducing sugars (30 g/L) compared with the other methods applied. The results showed that increasing the concentration of H2SO4 up to 3% decreased the amounts of generated reducing sugars to 20.4 g/L, which is about 32% less. The fermentation of 1%, 2%, and 3% SAPKC resulted in the production of ABE of 1.07, 5.72, and 3.48 g/L, respectively. This study showed that the pretreatment of PKC improved the content of fermentable sugars and subsequently enhanced the production of ABE by C. acetobutylicum YM1. This study also revealed that PKC can be regarded as a potentially low cost substrate for ABE fermentation.

Original languageEnglish
Pages (from-to)3371-3386
Number of pages16
JournalBioResources
Volume12
Issue number2
DOIs
Publication statusPublished - 1 Jan 2017

Fingerprint

Clostridium
Butanols
Acetone
acetone
Butenes
Fermentation
fermentation
ethanol
Ethanol
Sugars
sugar
sulfuric acid
Sodium Hydroxide
Palm oil
Enzymatic hydrolysis
Acids
Hydrochloric Acid
Peroxides
Autoclaves
Alkalies

Keywords

  • Acetone-butanol-ethanol (ABE)
  • Clostridium acetobutylicum YM1
  • Palm kernel cake (PKC)
  • Pretreatment

ASJC Scopus subject areas

  • Environmental Engineering
  • Bioengineering
  • Waste Management and Disposal

Cite this

Production of Acetone, Butanol, and Ethanol (ABE) by clostridium acetobutylicum YM1 from pretreated palm kernel cake in batch culture fermentation. / Al-Tabib, Abdualati Ibrahim; Al-Shorgani, Najeeb Kaid Nasser; Abu Hasan, Hassimi; Abdul Hamid, Aidil; Kalil, Mohd. Sahaid.

In: BioResources, Vol. 12, No. 2, 01.01.2017, p. 3371-3386.

Research output: Contribution to journalArticle

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abstract = "The viability of most fermentation processes is very much dependent on the cheap fermentation medium used. Palm kernel cake (PKC) is an abundant biomass generated from the palm oil processing industry that can be used as the carbon source for the growth and production of acetone-butanol-ethanol fermentation (ABE) by Clostridia. In this study, ABE production from the fermentation of PKC using Clostridium acetobutylicum YM1 in a batch culture was conducted. The PKC was subjected to treatment with acids (sulphuric and hydrochloric acids), alkali (sodium hydroxide and alkaline peroxide), enzymatic hydrolysis, and hydrothermal treatment (in autoclave). The sulphuric acid-treated PKC (2{\%} SAPKC) method produced the highest concentration of reducing sugars (30 g/L) compared with the other methods applied. The results showed that increasing the concentration of H2SO4 up to 3{\%} decreased the amounts of generated reducing sugars to 20.4 g/L, which is about 32{\%} less. The fermentation of 1{\%}, 2{\%}, and 3{\%} SAPKC resulted in the production of ABE of 1.07, 5.72, and 3.48 g/L, respectively. This study showed that the pretreatment of PKC improved the content of fermentable sugars and subsequently enhanced the production of ABE by C. acetobutylicum YM1. This study also revealed that PKC can be regarded as a potentially low cost substrate for ABE fermentation.",
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