Production and characterisation of anti-Burkholderia pseudomallei exotoxin monoclonal antibodies

Sheila Nathan, R. Mohamed, R. Pillai, L. K. Yong, Mohammed Noor Embi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hybridoma fusion and subsequent limited dilution cloning generated monoclonal antibodies towards the exotoxin of Burkholderia pseudomallei. Ten subclones were produced from three parent hybridomas and one representative subclone of each parent was further characterised. All subclones demonstrated high binding activity towards the exotoxin as shown by ELISA with almost no reactivity towards the other extracellular products of B. pseudomallei. Isotyping studies revealed that the major antibody class was either IgG or IgA. Further characterisation of the antibodies by Western blotting demonstrated that two clones had a binding specificity towards only the 37 kD subunit of the exotoxin whilst the third clone hybridised with two discrete and distinct bands at the 37 and 17 kD subunits. Nonetheless, the monoclonal antibodies secreted by the subclones were able to only partially neutralise the in vitro cytotoxic activity of the B. pseudomallei exotoxin.

Original languageEnglish
Pages (from-to)17-25
Number of pages9
JournalJournal of Biochemistry, Molecular Biology and Biophysics
Volume4
Issue number1
Publication statusPublished - 2000

Fingerprint

Burkholderia pseudomallei
Exotoxins
Monoclonal Antibodies
Hybridomas
Clone Cells
Immunoglobulin Isotypes
Cloning
Immunoglobulin A
Dilution
Organism Cloning
Fusion reactions
Immunoglobulin G
Western Blotting
Enzyme-Linked Immunosorbent Assay
Antibodies

Keywords

  • Burkholderia pseudomallei
  • Exotoxin
  • Monoclonal antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Molecular Biology

Cite this

Production and characterisation of anti-Burkholderia pseudomallei exotoxin monoclonal antibodies. / Nathan, Sheila; Mohamed, R.; Pillai, R.; Yong, L. K.; Embi, Mohammed Noor.

In: Journal of Biochemistry, Molecular Biology and Biophysics, Vol. 4, No. 1, 2000, p. 17-25.

Research output: Contribution to journalArticle

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