Processing optimization and characterization of gelatin from catfish (Clarias gariepinus) skin

V. Sanaei Ardekani, F. Mahmoodani, S. F. See, Salma Mohamad Yusop, Abd. Salam Babji

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The extraction of catfish (Clarias gariepinus) skin gelatin was optimized by using response surface methodology (RSM) employing a central composite design (CCD). RSM with 3-factors, 5-levels CCD was carried out for the optimization. The independent variables were suggested which include NaOH concentration (0.07-0.23 mol/L), acetic acid concentration (0.04-0.14 mol/L) and extraction temperature (40-80°C) with the percentage of hydroxyproline recovery (Y) as dependant variable. A maximum Y of 65.32% for gelatin processing was obtained using a combination of 0.13 mol/L NaOH and 0.09 mol/L acetic acid for 1 h, followed by a hot-water extraction at 64.92°C for 3 h. The results indicated a high protein content (88.46 g/100 g) in the extracted gelatin with a viscosity of 3.45 mPa.s, 286.71 g gel strength and 173 residues per 1000 residues of imino acids (proline and hydroxyproline). Furthermore, the gelatin from catfish also showed a relatively good instrumental texture quality according to texture profile analysis (TPA).

Original languageEnglish
Pages (from-to)1697-1705
Number of pages9
JournalSains Malaysiana
Volume42
Issue number12
Publication statusPublished - Dec 2013

Fingerprint

Clarias gariepinus
gelatin
skin (animal)
catfish
hydroxyproline
response surface methodology
acetic acid
texture
gel strength
proline
viscosity
protein content
temperature
water

Keywords

  • Bovine gelatin
  • Catfish
  • Central composite design
  • Fish skin gelatin
  • Response surface methodology

ASJC Scopus subject areas

  • General

Cite this

Processing optimization and characterization of gelatin from catfish (Clarias gariepinus) skin. / Ardekani, V. Sanaei; Mahmoodani, F.; See, S. F.; Mohamad Yusop, Salma; Babji, Abd. Salam.

In: Sains Malaysiana, Vol. 42, No. 12, 12.2013, p. 1697-1705.

Research output: Contribution to journalArticle

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