Process optimization of butanol production by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) using palm oil mill effluent in acetone-butanol-ethanol fermentation

Najeeb Kaid Nasser Al-Shorgani, Hafiza Shukor, Peyman Abdeshahian, Mohd Yusuf Mohd Nazir, Mohd. Sahaid Kalil, Aidil Abdul Hamid, Wan Mohtar Wan Yusoff

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Palm oil mill effluent (POME), an abundant and low-cost agro-industrial residue, was utilized for the production of butanol in the anaerobic process of acetone-butanol-ethanol (ABE) fermentation. The ABE process was performed by the cultivation of Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in raw POME medium without supplementation. The effect of various process parameters, including pH, incubation temperature, agitation and inoculums size on butanol production, was studied. Experimental results showed that the highest butanol levels could be produced when the initial pH of culture medium, incubation temperature, agitation speed and inoculums size were set at 5.8, 30. °C, 100. rpm and 15% (v/v), respectively. Results revealed that the existence of crude palm oil (CPO) in the POME composition had an adverse effect on butanol synthesis by Clostridial cells. The highest butanol and ABE concentrations of 0.9. g/L and 2.09. g/L were produced, respectively, when operating variables tested were set at their optimum levels under the absence of CPO.

Original languageEnglish
Pages (from-to)244-249
Number of pages6
JournalBiocatalysis and Agricultural Biotechnology
Volume4
Issue number2
DOIs
Publication statusPublished - 1 Apr 2015

Fingerprint

Clostridium saccharoperbutylacetonicum
oil mill effluents
ethanol fermentation
Clostridium
Butanols
Palm oil
butanol
Acetone
Butenes
Fermentation
acetone
Effluents
Ethanol
Petroleum
agitation
palm oils
ethanol
Temperature
palm oil
Elaeis guineensis

Keywords

  • ABE fermentation
  • Butanol production
  • Clostridium saccharoperbutylacetonicum N1-4
  • Palm oil mill effluent (POME)

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Process optimization of butanol production by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) using palm oil mill effluent in acetone-butanol-ethanol fermentation. / Al-Shorgani, Najeeb Kaid Nasser; Shukor, Hafiza; Abdeshahian, Peyman; Mohd Nazir, Mohd Yusuf; Kalil, Mohd. Sahaid; Abdul Hamid, Aidil; Wan Yusoff, Wan Mohtar.

In: Biocatalysis and Agricultural Biotechnology, Vol. 4, No. 2, 01.04.2015, p. 244-249.

Research output: Contribution to journalArticle

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abstract = "Palm oil mill effluent (POME), an abundant and low-cost agro-industrial residue, was utilized for the production of butanol in the anaerobic process of acetone-butanol-ethanol (ABE) fermentation. The ABE process was performed by the cultivation of Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in raw POME medium without supplementation. The effect of various process parameters, including pH, incubation temperature, agitation and inoculums size on butanol production, was studied. Experimental results showed that the highest butanol levels could be produced when the initial pH of culture medium, incubation temperature, agitation speed and inoculums size were set at 5.8, 30. °C, 100. rpm and 15{\%} (v/v), respectively. Results revealed that the existence of crude palm oil (CPO) in the POME composition had an adverse effect on butanol synthesis by Clostridial cells. The highest butanol and ABE concentrations of 0.9. g/L and 2.09. g/L were produced, respectively, when operating variables tested were set at their optimum levels under the absence of CPO.",
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