Probing protein-protein interactions in real time

Mario B. Viani, Lia I. Pietrasanta, James B. Thompson, Ami Chand, Ilse C. Gebeshuber, Johannes H. Kindt, Michael Richter, Helen G. Hansma, Paul K. Hansma

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

We have used a prototype small cantilever atomic force microscope to observe, in real time, the interactions between individual protein molecules. In particular, we have observed individual molecules of the chaperonin protein GroES binding to and then dissociating from individual GroEL proteins, which were immobilized on a mica support. This work suggests that the small cantilever atomic force microscope is a useful tool for studying protein dynamics at the single molecule level.

Original languageEnglish
Pages (from-to)644-647
Number of pages4
JournalNature Structural Biology
Volume7
Issue number8
DOIs
Publication statusPublished - Aug 2000
Externally publishedYes

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Chaperonins
Chaperonin 60
Molecules
Microscopes
Protein Binding
Proteins
mica

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

Viani, M. B., Pietrasanta, L. I., Thompson, J. B., Chand, A., Gebeshuber, I. C., Kindt, J. H., ... Hansma, P. K. (2000). Probing protein-protein interactions in real time. Nature Structural Biology, 7(8), 644-647. https://doi.org/10.1038/77936

Probing protein-protein interactions in real time. / Viani, Mario B.; Pietrasanta, Lia I.; Thompson, James B.; Chand, Ami; Gebeshuber, Ilse C.; Kindt, Johannes H.; Richter, Michael; Hansma, Helen G.; Hansma, Paul K.

In: Nature Structural Biology, Vol. 7, No. 8, 08.2000, p. 644-647.

Research output: Contribution to journalArticle

Viani, MB, Pietrasanta, LI, Thompson, JB, Chand, A, Gebeshuber, IC, Kindt, JH, Richter, M, Hansma, HG & Hansma, PK 2000, 'Probing protein-protein interactions in real time', Nature Structural Biology, vol. 7, no. 8, pp. 644-647. https://doi.org/10.1038/77936
Viani MB, Pietrasanta LI, Thompson JB, Chand A, Gebeshuber IC, Kindt JH et al. Probing protein-protein interactions in real time. Nature Structural Biology. 2000 Aug;7(8):644-647. https://doi.org/10.1038/77936
Viani, Mario B. ; Pietrasanta, Lia I. ; Thompson, James B. ; Chand, Ami ; Gebeshuber, Ilse C. ; Kindt, Johannes H. ; Richter, Michael ; Hansma, Helen G. ; Hansma, Paul K. / Probing protein-protein interactions in real time. In: Nature Structural Biology. 2000 ; Vol. 7, No. 8. pp. 644-647.
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