Prevalence and antibiotic resistance of Salmonella Enteritidis and Salmonella Typhimurium in raw chicken meat at retail markets in Malaysia

T. Y. Thung, N. A. Mahyudin, Dayang Fredalina Basri, C. W J Wan Mohamed Radzi, Y. Nakaguchi, M. Nishibuchi, S. Radu

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Salmonellosis is one of the major food-borne diseases in many countries. This study was carried out to determine the occurrence of Salmonella spp., Salmonella Enteritidis, and Salmonella Typhimurium in raw chicken meat from wet markets and hypermarkets in Selangor, as well as to determine the antibiotic susceptibility profile of S. Enteritidis and S. Typhimurium. The most probable number (MPN) in combination with multiplex polymerase chain reaction (mPCR) method was used to quantify the Salmonella spp., S. Enteritidis, and S. Typhimurium in the samples. The occurrence of Salmonella spp., S. Enteritidis, and S. Typhimurium in 120 chicken meat samples were 20.80%, 6.70%, and 2.50%, respectively with estimated quantity varying from <3 to 15 MPN/g. The antibiogram testing revealed differential multi-drug resistance among S. Enteritidis and S. Typhimurium isolates. All the isolates were resistance to erythromycin, penicillin, and vancomycin whereas sensitivity was recorded for Amoxicillin/Clavulanic acid, Gentamicin, Tetracycline, and Trimethoprim. Our findings demonstrated that the retail chicken meat could be a source of multiple antimicrobial-resistance Salmonella and may constitute a public health concern in Malaysia.

Original languageEnglish
Pages (from-to)1888-1893
Number of pages6
JournalPoultry Science
Volume95
Issue number8
DOIs
Publication statusPublished - 1 Aug 2016

Fingerprint

raw chicken meat
retail marketing
Salmonella Enteritidis
Salmonella Typhimurium
antibiotic resistance
Malaysia
Salmonella
chicken meat
clavulanic acid
vancomycin
amoxicillin
multiple drug resistance
trimethoprim
erythromycin
salmonellosis
penicillins
gentamicin
foodborne illness
tetracycline
public health

Keywords

  • antimicrobial susceptibility
  • chicken meat
  • most probable number PCR
  • prevalence
  • Salmonella

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Prevalence and antibiotic resistance of Salmonella Enteritidis and Salmonella Typhimurium in raw chicken meat at retail markets in Malaysia. / Thung, T. Y.; Mahyudin, N. A.; Basri, Dayang Fredalina; Wan Mohamed Radzi, C. W J; Nakaguchi, Y.; Nishibuchi, M.; Radu, S.

In: Poultry Science, Vol. 95, No. 8, 01.08.2016, p. 1888-1893.

Research output: Contribution to journalArticle

Thung, T. Y. ; Mahyudin, N. A. ; Basri, Dayang Fredalina ; Wan Mohamed Radzi, C. W J ; Nakaguchi, Y. ; Nishibuchi, M. ; Radu, S. / Prevalence and antibiotic resistance of Salmonella Enteritidis and Salmonella Typhimurium in raw chicken meat at retail markets in Malaysia. In: Poultry Science. 2016 ; Vol. 95, No. 8. pp. 1888-1893.
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