Pressure drop correlations and scale-up of size exclusion chromatography with compressible packings

Abdul Wahab Mohammad, Donna G. Stevenson, Phillip C. Wankat

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Pressure drop measurements were made for compressible gel packings Sephadex G-25 and G-100. A critical velocity at which pressure drop kept increasing was observed. Correlations were developed for pressure drop. Measurements of the chromatographic separation obtained for separating bovine serum albumin and nickel nitrate were correlated as measurements of H. A scaling procedure which keeps separation, pressure drop, and feed throughput constant was developed for each packing. The procedure and results for G-25 are similar to those developed previously for rigid packings. The use of smaller diameter packings can greatly reduce the amount of packing required. For G-100, which is quite compressible, the scaling procedure was different. Results of the scaling analysis for G-100 showed that there is little advantage to decreasing the particle size with very compressible packings.

Original languageEnglish
Pages (from-to)549-561
Number of pages13
JournalIndustrial and Engineering Chemistry Research
Volume31
Issue number2
Publication statusPublished - Feb 1992
Externally publishedYes

Fingerprint

Size exclusion chromatography
pressure drop
chromatography
Pressure drop
Bovine Serum Albumin
serum
Nitrates
nickel
Gels
gel
Particle size
Nickel
particle size
Throughput
nitrate

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Environmental Science(all)
  • Polymers and Plastics

Cite this

Pressure drop correlations and scale-up of size exclusion chromatography with compressible packings. / Mohammad, Abdul Wahab; Stevenson, Donna G.; Wankat, Phillip C.

In: Industrial and Engineering Chemistry Research, Vol. 31, No. 2, 02.1992, p. 549-561.

Research output: Contribution to journalArticle

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