Preparation of Orthosiphon stamineus enriched-extracts and evaluation of their free radical scavenging activity

Che Nurul Ain Nadirah Che Mansor, Jalifah Latip, Masturah Markom

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Orthosiphon stamineus is one of the important herbal plants used in folk medicine to cure variety of diseases. Three compounds namely rosmarinic acid (RA), sinensetin (SEN) and eupatorin (EUP) were identified as the bioactive markers. However, a standardized extraction method for the preparation of O. stamineus extract enriched with the bioactive compounds was still undiscovered. Thus, this study aims to establish the optimal extraction method that can be used to prepare the enriched extract with anti-oxidant property. Maceration, reflux and Soxhlet were the extraction methods employed, with ethanol, 50% (v/v) aqueous ethanol and water were chosen as the solvents. Each extracts were evaluated for their biomarker contents (RA, SEN and EUP) and anti-oxidant capacity using thin layer chromatography (TLC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay respectively. Among the three extraction methods employed, the highest total extraction yield was obtained from reflux (72.73%) followed by Soxhlet (62.51%) and maceration (37.78%). Although all extracts found to contain the three biomarkers via TLC visualization analysis, there was variation in the extracts' anti-oxidant capacity, ranging from 6.17% to 72.97%. The variation was expected to be due to the difference in the quantity of the biomarkers in each extract. Furthermore, the anti-oxidative potency of the RA was found comparable to natural anti-oxidant vitamin C, and higher than the synthetic anti-oxidant butylated hydroxyl toluene (BHT). These preliminary results may serve as a starting point towards the preparation of standardized bioactive O. stamineus extract.

Original languageEnglish
Title of host publication2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1784
ISBN (Electronic)9780735414464
DOIs
Publication statusPublished - 17 Nov 2016
Event2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016 - Selangor, Malaysia
Duration: 13 Apr 201614 Apr 2016

Other

Other2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016
CountryMalaysia
CitySelangor
Period13/4/1614/4/16

Fingerprint

scavenging
free radicals
biomarkers
preparation
activity (biology)
evaluation
thin layer chromatography
acids
ethyl alcohol
ascorbic acid
medicine
markers
toluene
water

Keywords

  • DPPH
  • maceration
  • Orthosiphon stamineus
  • reflux
  • Soxhlet
  • TLC

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Che Mansor, C. N. A. N., Latip, J., & Markom, M. (2016). Preparation of Orthosiphon stamineus enriched-extracts and evaluation of their free radical scavenging activity. In 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium (Vol. 1784). [030013] American Institute of Physics Inc.. https://doi.org/10.1063/1.4966751

Preparation of Orthosiphon stamineus enriched-extracts and evaluation of their free radical scavenging activity. / Che Mansor, Che Nurul Ain Nadirah; Latip, Jalifah; Markom, Masturah.

2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784 American Institute of Physics Inc., 2016. 030013.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Che Mansor, CNAN, Latip, J & Markom, M 2016, Preparation of Orthosiphon stamineus enriched-extracts and evaluation of their free radical scavenging activity. in 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. vol. 1784, 030013, American Institute of Physics Inc., 2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016, Selangor, Malaysia, 13/4/16. https://doi.org/10.1063/1.4966751
Che Mansor CNAN, Latip J, Markom M. Preparation of Orthosiphon stamineus enriched-extracts and evaluation of their free radical scavenging activity. In 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784. American Institute of Physics Inc. 2016. 030013 https://doi.org/10.1063/1.4966751
Che Mansor, Che Nurul Ain Nadirah ; Latip, Jalifah ; Markom, Masturah. / Preparation of Orthosiphon stamineus enriched-extracts and evaluation of their free radical scavenging activity. 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784 American Institute of Physics Inc., 2016.
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abstract = "Orthosiphon stamineus is one of the important herbal plants used in folk medicine to cure variety of diseases. Three compounds namely rosmarinic acid (RA), sinensetin (SEN) and eupatorin (EUP) were identified as the bioactive markers. However, a standardized extraction method for the preparation of O. stamineus extract enriched with the bioactive compounds was still undiscovered. Thus, this study aims to establish the optimal extraction method that can be used to prepare the enriched extract with anti-oxidant property. Maceration, reflux and Soxhlet were the extraction methods employed, with ethanol, 50{\%} (v/v) aqueous ethanol and water were chosen as the solvents. Each extracts were evaluated for their biomarker contents (RA, SEN and EUP) and anti-oxidant capacity using thin layer chromatography (TLC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay respectively. Among the three extraction methods employed, the highest total extraction yield was obtained from reflux (72.73{\%}) followed by Soxhlet (62.51{\%}) and maceration (37.78{\%}). Although all extracts found to contain the three biomarkers via TLC visualization analysis, there was variation in the extracts' anti-oxidant capacity, ranging from 6.17{\%} to 72.97{\%}. The variation was expected to be due to the difference in the quantity of the biomarkers in each extract. Furthermore, the anti-oxidative potency of the RA was found comparable to natural anti-oxidant vitamin C, and higher than the synthetic anti-oxidant butylated hydroxyl toluene (BHT). These preliminary results may serve as a starting point towards the preparation of standardized bioactive O. stamineus extract.",
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