Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coli BL21

Siti Syazwani Mohd Shah, Abdullah Amru Indera Luthfi, Kheng Oon Low, Shuhaida Harun, Shareena Fairuz Abdul Manaf, Rosli Md Illias, Jamaliah Md Jahim

Research output: Contribution to journalArticle

Abstract

Kenaf (Hibiscus cannabinus L.), a potential fibre crop with a desirably high growth rate, could serve as a sustainable feedstock in the production of xylitol. In this work, the extraction of soluble products of kenaf through dilute nitric-acid hydrolysis was elucidated with respect to three parameters, namely temperature, residence time, and acid concentration. The study will assist in evaluating the performance in terms of xylose recovery. The result point out that the maximum xylose yield of 30.7 g per 100 g of dry kenaf was attained from 2% (v/v) HNO 3 at 130 °C for 60 min. The detoxified hydrolysate was incorporated as the primary carbon source for subsequent fermentation by recombinant Escherichia coli and the performance of strain on five different semi-synthetic media on xylitol production were evaluated herein. Among these media, batch cultivation in a basal salt medium (BSM) afforded the highest xylitol yield of 0.35 g/g based on xylose consumption, which corresponded to 92.8% substrate utilization after 38 h. Subsequently, fermentation by E. coli in the xylose-based kenaf hydrolysate supplemented with BSM resulting in 6.8 g/L xylitol which corresponding to xylitol yield of 0.38 g/g. These findings suggested that the use of kenaf as the fermentation feedstock could be advantageous for the development of sustainable xylitol production.

Original languageEnglish
Article number4080
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

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Hibiscus
Xylitol
Xylose
Escherichia coli
Fermentation
Salts
Nitric Acid
Conservation of Natural Resources
Hydrolysis
Carbon
Temperature
Acids
Growth

ASJC Scopus subject areas

  • General

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Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coli BL21. / Shah, Siti Syazwani Mohd; Luthfi, Abdullah Amru Indera; Low, Kheng Oon; Harun, Shuhaida; Manaf, Shareena Fairuz Abdul; Illias, Rosli Md; Md Jahim, Jamaliah.

In: Scientific Reports, Vol. 9, No. 1, 4080, 01.12.2019.

Research output: Contribution to journalArticle

Shah, Siti Syazwani Mohd ; Luthfi, Abdullah Amru Indera ; Low, Kheng Oon ; Harun, Shuhaida ; Manaf, Shareena Fairuz Abdul ; Illias, Rosli Md ; Md Jahim, Jamaliah. / Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coli BL21. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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