Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactide-co-glycolide) microspheres

Rezaul H. Ansary, Mokhlesur M. Rahman, Mohamed B. Awang, Haliza Katas, Hazrina Hadi, Farahidah Mohamed, Abd Almonem Doolaanea, Yunus B. Kamaruzzaman

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst release and to eliminate the lag phase from the release profile of PLGA microspheres. The double-walled microspheres were prepared using a modified water-in-oil-in-oil-in-water (w/o/o/w) method and single-polymer microspheres were prepared using a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The particle size, morphology, encapsulation efficiency, thermal properties, in vitro drug release and structural integrity of BSA were evaluated in this study. Double-walled microspheres prepared with Glu-PLGA and PLGA polymers with a mass ratio of 1:1 were non-porous, smooth-surfaced, and spherical in shape. A significant reduction of initial burst release was achieved for the double-walled microspheres compared to single-polymer microspheres. In addition, microspheres prepared using Glu-PLGA and PLGA polymers in a mass ratio of 1:1 exhibited continuous BSA release after the small initial burst without any lag phase. It can be concluded that the double-walled microspheres made of Glu-PLGA and PLGA polymers in a mass ratio of 1:1 can be a potential delivery system for pharmaceutical proteins.

Original languageEnglish
Pages (from-to)1-15
Number of pages15
JournalArchives of Pharmacal Research
DOIs
Publication statusAccepted/In press - 27 Jan 2016

Fingerprint

Polyglactin 910
Bovine Serum Albumin
Microspheres
Glucose
Polymers
Oils
Water
polylactic acid-polyglycolic acid copolymer
In Vitro Techniques
Structural integrity
Emulsions
Encapsulation
Particle Size
Pharmaceutical Preparations
Hydroxyl Radical
Evaporation
Proteins
Thermodynamic properties
Hot Temperature
Particle size

Keywords

  • Controlled release
  • Drug delivery
  • Encapsulation efficiency
  • Microspheres
  • Poly(lactide-co-glycolide)
  • Therapeutic proteins

ASJC Scopus subject areas

  • Drug Discovery
  • Molecular Medicine
  • Organic Chemistry

Cite this

Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactide-co-glycolide) microspheres. / Ansary, Rezaul H.; Rahman, Mokhlesur M.; Awang, Mohamed B.; Katas, Haliza; Hadi, Hazrina; Mohamed, Farahidah; Doolaanea, Abd Almonem; Kamaruzzaman, Yunus B.

In: Archives of Pharmacal Research, 27.01.2016, p. 1-15.

Research output: Contribution to journalArticle

Ansary, Rezaul H. ; Rahman, Mokhlesur M. ; Awang, Mohamed B. ; Katas, Haliza ; Hadi, Hazrina ; Mohamed, Farahidah ; Doolaanea, Abd Almonem ; Kamaruzzaman, Yunus B. / Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactide-co-glycolide) microspheres. In: Archives of Pharmacal Research. 2016 ; pp. 1-15.
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AU - Ansary, Rezaul H.

AU - Rahman, Mokhlesur M.

AU - Awang, Mohamed B.

AU - Katas, Haliza

AU - Hadi, Hazrina

AU - Mohamed, Farahidah

AU - Doolaanea, Abd Almonem

AU - Kamaruzzaman, Yunus B.

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N2 - The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst release and to eliminate the lag phase from the release profile of PLGA microspheres. The double-walled microspheres were prepared using a modified water-in-oil-in-oil-in-water (w/o/o/w) method and single-polymer microspheres were prepared using a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The particle size, morphology, encapsulation efficiency, thermal properties, in vitro drug release and structural integrity of BSA were evaluated in this study. Double-walled microspheres prepared with Glu-PLGA and PLGA polymers with a mass ratio of 1:1 were non-porous, smooth-surfaced, and spherical in shape. A significant reduction of initial burst release was achieved for the double-walled microspheres compared to single-polymer microspheres. In addition, microspheres prepared using Glu-PLGA and PLGA polymers in a mass ratio of 1:1 exhibited continuous BSA release after the small initial burst without any lag phase. It can be concluded that the double-walled microspheres made of Glu-PLGA and PLGA polymers in a mass ratio of 1:1 can be a potential delivery system for pharmaceutical proteins.

AB - The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst release and to eliminate the lag phase from the release profile of PLGA microspheres. The double-walled microspheres were prepared using a modified water-in-oil-in-oil-in-water (w/o/o/w) method and single-polymer microspheres were prepared using a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The particle size, morphology, encapsulation efficiency, thermal properties, in vitro drug release and structural integrity of BSA were evaluated in this study. Double-walled microspheres prepared with Glu-PLGA and PLGA polymers with a mass ratio of 1:1 were non-porous, smooth-surfaced, and spherical in shape. A significant reduction of initial burst release was achieved for the double-walled microspheres compared to single-polymer microspheres. In addition, microspheres prepared using Glu-PLGA and PLGA polymers in a mass ratio of 1:1 exhibited continuous BSA release after the small initial burst without any lag phase. It can be concluded that the double-walled microspheres made of Glu-PLGA and PLGA polymers in a mass ratio of 1:1 can be a potential delivery system for pharmaceutical proteins.

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