Abstract
The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst release and to eliminate the lag phase from the release profile of PLGA microspheres. The double-walled microspheres were prepared using a modified water-in-oil-in-oil-in-water (w/o/o/w) method and single-polymer microspheres were prepared using a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The particle size, morphology, encapsulation efficiency, thermal properties, in vitro drug release and structural integrity of BSA were evaluated in this study. Double-walled microspheres prepared with Glu-PLGA and PLGA polymers with a mass ratio of 1:1 were non-porous, smooth-surfaced, and spherical in shape. A significant reduction of initial burst release was achieved for the double-walled microspheres compared to single-polymer microspheres. In addition, microspheres prepared using Glu-PLGA and PLGA polymers in a mass ratio of 1:1 exhibited continuous BSA release after the small initial burst without any lag phase. It can be concluded that the double-walled microspheres made of Glu-PLGA and PLGA polymers in a mass ratio of 1:1 can be a potential delivery system for pharmaceutical proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 1-15 |
| Number of pages | 15 |
| Journal | Archives of Pharmacal Research |
| DOIs | |
| Publication status | Accepted/In press - 27 Jan 2016 |
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Keywords
- Controlled release
- Drug delivery
- Encapsulation efficiency
- Microspheres
- Poly(lactide-co-glycolide)
- Therapeutic proteins
ASJC Scopus subject areas
- Drug Discovery
- Molecular Medicine
- Organic Chemistry
Cite this
Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactide-co-glycolide) microspheres. / Ansary, Rezaul H.; Rahman, Mokhlesur M.; Awang, Mohamed B.; Katas, Haliza; Hadi, Hazrina; Mohamed, Farahidah; Doolaanea, Abd Almonem; Kamaruzzaman, Yunus B.
In: Archives of Pharmacal Research, 27.01.2016, p. 1-15.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactide-co-glycolide) microspheres
AU - Ansary, Rezaul H.
AU - Rahman, Mokhlesur M.
AU - Awang, Mohamed B.
AU - Katas, Haliza
AU - Hadi, Hazrina
AU - Mohamed, Farahidah
AU - Doolaanea, Abd Almonem
AU - Kamaruzzaman, Yunus B.
PY - 2016/1/27
Y1 - 2016/1/27
N2 - The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst release and to eliminate the lag phase from the release profile of PLGA microspheres. The double-walled microspheres were prepared using a modified water-in-oil-in-oil-in-water (w/o/o/w) method and single-polymer microspheres were prepared using a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The particle size, morphology, encapsulation efficiency, thermal properties, in vitro drug release and structural integrity of BSA were evaluated in this study. Double-walled microspheres prepared with Glu-PLGA and PLGA polymers with a mass ratio of 1:1 were non-porous, smooth-surfaced, and spherical in shape. A significant reduction of initial burst release was achieved for the double-walled microspheres compared to single-polymer microspheres. In addition, microspheres prepared using Glu-PLGA and PLGA polymers in a mass ratio of 1:1 exhibited continuous BSA release after the small initial burst without any lag phase. It can be concluded that the double-walled microspheres made of Glu-PLGA and PLGA polymers in a mass ratio of 1:1 can be a potential delivery system for pharmaceutical proteins.
AB - The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst release and to eliminate the lag phase from the release profile of PLGA microspheres. The double-walled microspheres were prepared using a modified water-in-oil-in-oil-in-water (w/o/o/w) method and single-polymer microspheres were prepared using a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The particle size, morphology, encapsulation efficiency, thermal properties, in vitro drug release and structural integrity of BSA were evaluated in this study. Double-walled microspheres prepared with Glu-PLGA and PLGA polymers with a mass ratio of 1:1 were non-porous, smooth-surfaced, and spherical in shape. A significant reduction of initial burst release was achieved for the double-walled microspheres compared to single-polymer microspheres. In addition, microspheres prepared using Glu-PLGA and PLGA polymers in a mass ratio of 1:1 exhibited continuous BSA release after the small initial burst without any lag phase. It can be concluded that the double-walled microspheres made of Glu-PLGA and PLGA polymers in a mass ratio of 1:1 can be a potential delivery system for pharmaceutical proteins.
KW - Controlled release
KW - Drug delivery
KW - Encapsulation efficiency
KW - Microspheres
KW - Poly(lactide-co-glycolide)
KW - Therapeutic proteins
UR - http://www.scopus.com/inward/record.url?scp=84955585703&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84955585703&partnerID=8YFLogxK
U2 - 10.1007/s12272-016-0710-3
DO - 10.1007/s12272-016-0710-3
M3 - Article
C2 - 26818028
AN - SCOPUS:84955585703
SP - 1
EP - 15
JO - Archives of Pharmacal Research
JF - Archives of Pharmacal Research
SN - 0253-6269
ER -