Preparation and characterization of silver nanoparticles-reduced graphene oxide on ITO for immunosensing platform

A. Jamil, H. N. Lim, N. A. Yusof, A. Ahmad Tajudin, N. M. Huang, A. Pandikumar, A. Moradi Golsheikh, Yook Heng Lee, Y. Andou

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We here report the in-situ electrochemical deposition of silver nanoparticles (AgNPs) and a reduced graphene oxide (rGO) nanocomposite on the surface of an indium tin oxide (ITO) electrode. The nanocomposite modified ITO was used as a basic platform for the construction of a novel electrochemical biosensor for the detection of hydrogen peroxide (H2O2); where horseradish peroxidase (HRP) tagged antibodies acted as recognition elements for carcinoembryonic antigen (CEA). The AgNPs-rGO/ITO modified electrode remarkably outperformed a bare electrode because of its enhanced surface area and electrocatalytic activity, resulting in a notably amplified electrical signal for the detection of H2O2. Through a cyclic voltammetric (CV) technique, the detection limit of the sandwich type immunoassay configuration was found to be 214 μM, with a linear range of 25-500 μM, as compared to the non-sandwich system, which was 120 μM with the same linear range. In contrast, the current-time response of the resulting sandwich-type immunoassay configuration showed a wider linear response to H2O2 in the range of 25-1450 μM, with a detection limit of 5.3 μM, while the non-sandwich system exhibited a linear range of 25-1355 μM (R2 = 0.9992), with a detection limit of 10 μM. Therefore, the current-time response provided a more sensitive means of detecting H2O2. The resulting immunosensor also exhibited outstanding stability and reproducibility, as well as selectivity toward H2O2 in the presence of several interferences.

Original languageEnglish
Pages (from-to)1423-1432
Number of pages10
JournalSensors and Actuators, B: Chemical
Volume221
DOIs
Publication statusPublished - 24 Aug 2015

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Tin oxides
Silver
indium oxides
Indium
Oxides
Graphene
tin oxides
graphene
platforms
silver
Nanoparticles
nanoparticles
preparation
Electrodes
oxides
Nanocomposites
immunoassay
time response
Immunosensors

Keywords

  • Biosensor
  • ELISA
  • Graphene
  • Immunosensor
  • Silver nanoparticles

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Metals and Alloys
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Instrumentation

Cite this

Preparation and characterization of silver nanoparticles-reduced graphene oxide on ITO for immunosensing platform. / Jamil, A.; Lim, H. N.; Yusof, N. A.; Tajudin, A. Ahmad; Huang, N. M.; Pandikumar, A.; Golsheikh, A. Moradi; Lee, Yook Heng; Andou, Y.

In: Sensors and Actuators, B: Chemical, Vol. 221, 24.08.2015, p. 1423-1432.

Research output: Contribution to journalArticle

Jamil, A. ; Lim, H. N. ; Yusof, N. A. ; Tajudin, A. Ahmad ; Huang, N. M. ; Pandikumar, A. ; Golsheikh, A. Moradi ; Lee, Yook Heng ; Andou, Y. / Preparation and characterization of silver nanoparticles-reduced graphene oxide on ITO for immunosensing platform. In: Sensors and Actuators, B: Chemical. 2015 ; Vol. 221. pp. 1423-1432.
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