Prenatal diagnosis of aneuploidies in amniotic fluid by multiple ligation-dependent probe amplification (MLPA) analysis

Noor Hamidah Hussin, A. R. Munirah, Hafiza Alauddin, A. R. FARISAH, Shuhaila Ahmad, M. N. Norzilawati, M. Y. Jamil, O. Ainoon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Prenatal diagnosis is essential in the new era of diagnosis and management of genetic diseases in obstetrics. Multiple ligation-dependent probe amplification (MLPA) is a recent technique for prenatal diagnosis for the relative quantification of 40 different nucleic acid sequences in one single reaction. We had utilized the MLPA technique in detecting aneuploidies in amniotic fluid samples from 25 pregnant women from the Obstetrics and Gynaecology Department UKMMC, versus the quantitative fluorescent polymerase chain reaction (QF-PCR) method. Conclusive results were obtained in 18 cases and all were concordant with that of the QF-PCR. All four cases of trisomies were correctly identified including one case with maternal cell contamination.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalMalaysian Journal of Pathology
Volume36
Issue number3
Publication statusPublished - 1 Dec 2014

Fingerprint

Aneuploidy
Amniotic Fluid
Prenatal Diagnosis
Ligation
Polymerase Chain Reaction
Inborn Genetic Diseases
Hospital Obstetrics and Gynecology Department
Trisomy
Gynecology
Nucleic Acids
Obstetrics
Pregnant Women
Mothers

Keywords

  • Amniotic fluid
  • DNA
  • Multiple ligation probe amplification (MLPA)
  • PCR
  • Prenatal diagnosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology
  • Medicine(all)

Cite this

Prenatal diagnosis of aneuploidies in amniotic fluid by multiple ligation-dependent probe amplification (MLPA) analysis. / Hussin, Noor Hamidah; Munirah, A. R.; Alauddin, Hafiza; FARISAH, A. R.; Ahmad, Shuhaila; Norzilawati, M. N.; Jamil, M. Y.; Ainoon, O.

In: Malaysian Journal of Pathology, Vol. 36, No. 3, 01.12.2014, p. 163-168.

Research output: Contribution to journalArticle

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AU - Ahmad, Shuhaila

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