Kajian awalan terhadap kinetik pengekstrakan pepejal-cecair dan analisis komponen bio-aktif daun hibiscus rosa-sinensis

Translated title of the contribution: Preliminary study on kinetic solid-liquid extraction and bio-active components analysis of hibiscus rosa-sinensis leaves

Saiful Irwan Zubairi, Haizulizam Suradi, Syazwan Aizad Abdul Mutalib, Zetty Shafiqa Othman, Norshahida Bustaman, Wan Ros Maryana Wan Musa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Hibiscus rosa-sinensis or commonly known as 'Hibiscus' is a kind of decorative flowers which often grown in a subtropical countries. This plant is often used in the preparation of traditional medicine because of its pharmacological properties that are capable of treating number of health problems. The plant contains several essential bio-active substances and nutrients especially in its flowers and leaves. One of the bioactive substances is β-sitosterol which is abundant in the leaf crude extract. Hibiscus leaves water extract yielded mucilage which is widely used in lowering high body temperature due to fever (antipyretic). Therefore, the main objective of this paper was to determine the maximum concentration of mucilage and time of exhaustive extraction from fresh and dried leaves using a Peleq's mathematical model. Moreover, several analyses were conducted such as qualitative analysis to determine the presence of bioactive substances using high performance liquid chromatography (HPLC) and determination of functional groups by means of fourier transform infrared method (FTIR). Physical properties of the extracts were evaluated to determine its acididity and viscosity of the mucilage with respect to different shear stress. The result show that the extract of dried Hibiscusleaves exhibited high in its concentrations even though the achieved exhaustive extraction was relatively slow as compared to fresh leaves (p<0.05). Based on the HPLC analysis, the main bio-active substances of β-sitosterol was only existed in fresh leaves samples. The absence of β-sitosterol in dried leaves extract was caused by the loss of otherimportant bio-active substances which possess surfactantcapability, due to thermal degradation of drying process or the component itself was deteriorated during the pre-preparation drying process. Furthermore, the FTIR analysis shows that the same composition of theabsorption peaks for both extracts with the highest absorption of O-H bonds was observed. This proves that the content of β-sitosterol in both extracts was too small and the composition of other phytosterols was observed to dominate and influence the availability of β-sitosterol in the crude extract. Meanwhile, physical properties analysis of the both extracts was carried to show the effect of drying process on the observed bio-active components. The high acidity of the fresh leaf extract was attributed to the presence of all bio-active ingredients. Meanwhile for the dried leaf extract, a close neutral pH value was observed due to the bio-active decomposition which is acidic in nature and sensitive to heat.

Original languageUndefined/Unknown
Pages (from-to)43-57
Number of pages15
JournalMalaysian Journal of Analytical Sciences
Volume18
Issue number1
Publication statusPublished - 2014

Fingerprint

Kinetics
Liquids
Drying
High performance liquid chromatography
Complex Mixtures
Fourier transforms
Physical properties
Infrared radiation
Phytosterols
Antipyretics
Medical problems
Chemical analysis
Acidity
Functional groups
Nutrients
Medicine
Shear stress
Pyrolysis
gamma-sitosterol
Availability

Keywords

  • β-sitosterol
  • FTIR
  • Hibiscus rosa-sinensis
  • HPLC
  • Mucilage
  • Solid-liquid extraction
  • Viscosity

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Kajian awalan terhadap kinetik pengekstrakan pepejal-cecair dan analisis komponen bio-aktif daun hibiscus rosa-sinensis. / Zubairi, Saiful Irwan; Suradi, Haizulizam; Mutalib, Syazwan Aizad Abdul; Othman, Zetty Shafiqa; Bustaman, Norshahida; Wan Musa, Wan Ros Maryana.

In: Malaysian Journal of Analytical Sciences, Vol. 18, No. 1, 2014, p. 43-57.

Research output: Contribution to journalArticle

Zubairi, Saiful Irwan ; Suradi, Haizulizam ; Mutalib, Syazwan Aizad Abdul ; Othman, Zetty Shafiqa ; Bustaman, Norshahida ; Wan Musa, Wan Ros Maryana. / Kajian awalan terhadap kinetik pengekstrakan pepejal-cecair dan analisis komponen bio-aktif daun hibiscus rosa-sinensis. In: Malaysian Journal of Analytical Sciences. 2014 ; Vol. 18, No. 1. pp. 43-57.
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