Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation

Abidah Md Noh, Siti Hajar Mohamad, Jamaliah Md Jahim, Abdul Munir Abd. Murad, Farah Diba Abu Bakar, Nor Muhammad, Mahadi, Rosli Md Illias

Research output: Contribution to journalArticle

Abstract

This study was aimed to investigate the production of recombinant cellobiohydrolase (CBH) in static liquid culture (SLC) fermentation using newly developed recombinant of A. niger. Different environmental parameters and culture conditions were used to attain good cultivation of A. niger in SLC fermentation and highest level of CBH production. The fermentation results were evaluated from CBH production, total biomass, total protein and the residual main carbon source, maltose after 9 days of cultivation. The CBH production was found effectively enhanced under optimized cultivation temperature, initial pH, inoculums size and pre-shaked inoculums. Maximum biomass, protein concentration and enzyme activity were determined as 1.5 g dry weight/ml medium, 3.5 mg/ml and 40 U/ml respectively. The results also show that SLC could enhance production of the recombinant CBH.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalAustralian Journal of Basic and Applied Sciences
Volume6
Issue number1
Publication statusPublished - Jan 2012

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cellulose 1,4-beta-cellobiosidase
Aspergillus niger
fermentation
liquids
biomass
maltose
proteins
enzyme activity
carbon
temperature

Keywords

  • Aspergillus niger
  • Cellobiohydrolase production
  • Pre-shaked inoculums
  • Static liquid culture

ASJC Scopus subject areas

  • General

Cite this

Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation. / Noh, Abidah Md; Mohamad, Siti Hajar; Md Jahim, Jamaliah; Abd. Murad, Abdul Munir; Abu Bakar, Farah Diba; Muhammad, Nor; Mahadi; Illias, Rosli Md.

In: Australian Journal of Basic and Applied Sciences, Vol. 6, No. 1, 01.2012, p. 43-49.

Research output: Contribution to journalArticle

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