Preliminary study of biotransformation of aldehydes and ketones by clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

Marni Farhani Mansor, Mohd. Sahaid Kalil

Research output: Contribution to journalArticle

Abstract

The application of Clostridium species as a whole cell biocatalysis represents an attractive alternative to conventional chemical synthesis. Solvent-producing cultures of Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) might act as a promising biocatalyst toreduce a variety of the carbonyl groups to the corresponding alcohols through whole cell biotransformation process. The main objective of this work was to investigate the ability of C.saccharoperbutylacetonicum N1-4to biotransform some aldehydes and ketones into the corresponding alcohols. A screening of 13 substrates belonging to aldehydes and ketones group was performed to illustrate the ability on the production of alcohol via biotransformation using the growing cells of C. saccharoperbutylacetonicum N1-4. The experiment was carried out with initial substrates of concentration 2.5 mM, pH 6.8±0.2 and temperature 30°C Enzyme that catalyse this biotransformation might be dehydrogenase enzyme since it catalyses the reduction reaction of aldehydes and ketones to the corresponding alcohols. Gas chromatography analysis indicated that microbial biotransformation definitely represents a new attractive approach of C. saccharoperbutylacetonicum N1-4 since it revealed that butanal, pentanal andcyclohexanone were biotransformed into butanol, pentanol and cyclohexanol, respectively.

Original languageEnglish
Pages (from-to)179-182
Number of pages4
JournalBiotechnology
Volume12
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

Clostridium
Biotransformation
Ketones
Aldehydes
Alcohols
Enzymes
Cyclohexanols
Biocatalysis
Pentanols
Butanols
Substrates
Gas chromatography
Gas Chromatography
Screening
Oxidoreductases
Temperature
Experiments

Keywords

  • Clostridium saccharoperbutylacetonicum N1-4
  • Reduction of ketone and aldehyde and alcohol production
  • Whole cell biotransformation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Preliminary study of biotransformation of aldehydes and ketones by clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). / Mansor, Marni Farhani; Kalil, Mohd. Sahaid.

In: Biotechnology, Vol. 12, No. 4, 2013, p. 179-182.

Research output: Contribution to journalArticle

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