Preliminary screening of antibacterial activity using crude extracts of Hibiscus rosa sinensis

Sangeetha Arullappan, Zubaidah Zakaria, Dayang Fredalina Basri

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Hibiscus rosa sinensis, a member of the Malvaceae family, is widely cultivated in the tropics as an ornamental plant. It is often planted as a fence or hedge plant, and has several forms of flowers with varying colours. It is also used in traditional medicine to induce abortion, ease menstrual cramps, assist in childbirth and relieve headache, fever and inflammation. In this study, we evaluated the antibacterial activity of H. rosa sinesis extract using a disc diffusion method. Crude petroleum ether extract, ethyl acetate extract and methanol extract from the leaves, stems and flowers of the plant were prepared using a cold extraction technique. These extracts were tested at concentrations ranging from 4 mg/disc to 0.017 mg/disc against methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia. The petroleum ether extract from the leaves, stems and flowers and methanol extract from the leaves showed inhibition zones with diameters > 12 mm against MRSA. Overall, the petroleum ether extract from flowers at concentrations of 4 mg/disc and 2 mg/disc displayed the strongest inhibition zones of 18.6 ± 2.85 mm and 18.5 ± 0.29 mm, respectively, as compared to vancomycin (30 μg/ml), which did not differ significantly from the 18.0 ± 0.10 mm size of the vancomycin (30 μg/ml) inhibition zone (p < 0.05). In conclusion, H. rosa sinensis extract is a potential antibacterial agent for treating MRSA infection.

Original languageEnglish
Pages (from-to)109-118
Number of pages10
JournalTropical Life Sciences Research
Volume20
Issue number2
Publication statusPublished - 2009

Fingerprint

Hibiscus rosa-sinensis
Hibiscus
Methicillin
Complex Mixtures
Screening
Methicillin-Resistant Staphylococcus aureus
Vancomycin
screening
Methanol
extracts
Tropics
Fences
Malvaceae
Plant Stems
Muscle Cramp
petroleum
Escherichia coli
Medicine
flowers
ethers

Keywords

  • Antibacterial activity
  • Hibiscus rosa sinensis
  • Inhibition zone

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Preliminary screening of antibacterial activity using crude extracts of Hibiscus rosa sinensis. / Arullappan, Sangeetha; Zakaria, Zubaidah; Basri, Dayang Fredalina.

In: Tropical Life Sciences Research, Vol. 20, No. 2, 2009, p. 109-118.

Research output: Contribution to journalArticle

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