Preliminary screening for antimicrobial activity of the pulp of Canarium odontophyllum Miq. (Dabai) fruit

Dayang Fredalina Basri, Nursyuhaiba Saidi, Hashimawati Mahari, Shafikha Saari, Jacinta Santhanam

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Canarium odontophyllum Miq. or locally known as 'C. odontophyllum' in Sarawak, Malaysia, is one of the underutilized fruits consumed for their antioxidant properties by the local community in Borneo. The aim of the present study was to evaluate the antimicrobial activity of crude extracts from the pulp of C. odontophyllum against pathogenic microorganisms. The air dried flesh pulp of C. odontophyllum was extracted using four solvents with different polarity against two Gram-negative bacteria; Pseudomonas aeruginosa and Escherichia coli, two Gram-positive bacteria; Methicillin-susceptible Staphylococcus aureus, (MSSA) and Methicillin-Resistant Staphylococcus aureus (MRSA), three yeast species; Candida albicans ATCC 90028, Candida glabrata ATCC 64677 and Candida krusei ATCC 6258 and two filamentous fungus; Aspergillus fumigatus ATCC 204305 and Aspergillus niger ATCC 6275. The hexane, acetone, methanol and aqueous extracts of C. odontophyllum pulp at 25, 50, 75 and 100 mg/ml were screened for antimicrobial activity using agar well diffusion method. The yield of the C. odontophyllum was the highest when methanol was used as the extraction solvent (12.46 %) followed by acetone (8.72%) and hexane (6.22%). The lowest yield was recorded for the aqueous extract of C. odontophyllum (2.78%). These findings showed that all the crude extracts of C. odontophyllum were not active against any of the bacteria tested. Out of all the fungus studied, only C. glabrata was susceptible towards the C. odontophyllum pulp extract. The acetone extract displayed moderate antimicrobial activity against C. glabrata with inhibitory zone of 8.0 ± 0.00 mm at all tested concentrations whereas C. glabrata was susceptible towards hexane extract only at 100 mg/ml compared to the positive control (Gentamicin at 10 μg/ml) with inhibitory zone of 18.0 ± 1.41mm. The present study indicated that C. odontophyllum is a good source of obtaining an alternative phytotherapeutic agent which can be developed as antiyeast agent.

Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalGlobal Journal of Pharmacology
Volume8
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Candida glabrata
Fruit
Hexanes
Acetone
Malaysia
Complex Mixtures
Methanol
Fungi
Borneo
Methicillin
Aspergillus fumigatus
Aspergillus niger
Gram-Positive Bacteria
Methicillin-Resistant Staphylococcus aureus
Gentamicins
Gram-Negative Bacteria
Candida albicans
Candida
Pseudomonas aeruginosa
Agar

Keywords

  • Antimicrobial agar
  • Burseraceae
  • Canarium odontophyllum
  • Extraction yield
  • Well diffusion

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Preliminary screening for antimicrobial activity of the pulp of Canarium odontophyllum Miq. (Dabai) fruit. / Basri, Dayang Fredalina; Saidi, Nursyuhaiba; Mahari, Hashimawati; Saari, Shafikha; Santhanam, Jacinta.

In: Global Journal of Pharmacology, Vol. 8, No. 2, 2014, p. 213-220.

Research output: Contribution to journalArticle

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