Preliminary evaluation of various rapid influenza diagnostic test methods for the detection of the novel influenza A (H1N1) in Universiti Kebangsaan Malaysia Medical Centre

Zainol Rashid Noor Zetti, Noor Zetti Zainol Rashid, Wong Kon Ken, M. Haslina, I. Ilina

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We evaluated the performance of four rapid influenza diagnostic test methods (RIDT) compared to real-time reverse-transcription polymerase chain reaction (rRT-PCR), for the detection of the novel swine-origin influenza A (H1N1) virus (S-OIV) in August 2009. A total of 270 respiratory specimens were tested with rRT-PCR, where 74 of these were tested by BinaxNow® (Inverness), 80 by QuickVue® (Quidel), 37 by Influenza A Antigen Rapid Test (Rockeby Biomed) and 79 by Directigen™ (BD). The sensitivities ranged from 4.4% to 37.0%, specificities 90.9% to 100.0%, positive predictive values 75.0% to 100.0% and negative predictive values 32.3% to 75.0%. RIDT were able to detect S-OIV but the sensitivities were low. The limitations of RIDT must be considered when interpreting results for clinical management.

Original languageEnglish
Pages (from-to)27-30
Number of pages4
JournalMedical Journal of Malaysia
Volume65
Issue number1
Publication statusPublished - Mar 2010

Fingerprint

Malaysia
Routine Diagnostic Tests
Human Influenza
Reverse Transcription
H1N1 Subtype Influenza A Virus
Polymerase Chain Reaction
Antigens

Keywords

  • Detection
  • Diagnostic
  • Laboratory methods
  • Novel swine-origin influenza A (H1N1)
  • Performance
  • Rapid tests

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Preliminary evaluation of various rapid influenza diagnostic test methods for the detection of the novel influenza A (H1N1) in Universiti Kebangsaan Malaysia Medical Centre",
abstract = "We evaluated the performance of four rapid influenza diagnostic test methods (RIDT) compared to real-time reverse-transcription polymerase chain reaction (rRT-PCR), for the detection of the novel swine-origin influenza A (H1N1) virus (S-OIV) in August 2009. A total of 270 respiratory specimens were tested with rRT-PCR, where 74 of these were tested by BinaxNow{\circledR} (Inverness), 80 by QuickVue{\circledR} (Quidel), 37 by Influenza A Antigen Rapid Test (Rockeby Biomed) and 79 by Directigen™ (BD). The sensitivities ranged from 4.4{\%} to 37.0{\%}, specificities 90.9{\%} to 100.0{\%}, positive predictive values 75.0{\%} to 100.0{\%} and negative predictive values 32.3{\%} to 75.0{\%}. RIDT were able to detect S-OIV but the sensitivities were low. The limitations of RIDT must be considered when interpreting results for clinical management.",
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AU - Ilina, I.

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