Preeminent productivity of 1,3-propanediol by Clostridium butyricum JKT37 and the role of using calcium carbonate as pH neutraliser in glycerol fermentation

Zhao Kang Tee, Jamaliah Md Jahim, Jian Ping Tan, Byung Hong Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Calcium carbonate was evaluated as a replacement for the base during the fermentation of glycerol by a highly productive strain of 1,3-propanediol (PDO), viz., Clostridium butyricum JKT37. Due to its high specific growth rate (µmax = 0.53 h−1), 40 g/L of glycerol was completely converted into 19.6 g/L of PDO in merely 7 h of batch fermentation, leaving only acetate and butyrate as the by-products. The accumulation of these volatile fatty acids was circumvented with the addition of calcium carbonate as the pH neutraliser before the fermentation was inoculated. An optimal amount of 15 g/L of calcium carbonate was statistically determined from screening with various glycerol concentrations (20–120 g/L). By substituting potassium hydroxide with calcium carbonate as the pH neutraliser for fermentation in a bioreactor, a similar yield (YPDO/glycerol = 0.6 mol/mol) with a constant pH was achieved at the end of the fermentation.

Original languageEnglish
Pages (from-to)296-304
Number of pages9
JournalBioresource Technology
Volume233
DOIs
Publication statusPublished - 2017

Fingerprint

Clostridium
Calcium Carbonate
Calcium carbonate
Glycerol
calcium carbonate
Fermentation
fermentation
Productivity
productivity
Volatile fatty acids
Potassium hydroxide
Volatile Fatty Acids
Butyrates
Bioreactors
bioreactor
hydroxide
Byproducts
acetate
Screening
Acetates

Keywords

  • 1,3-Propanediol
  • Calcium carbonate
  • Fermentation
  • Isolation
  • pH neutraliser

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Preeminent productivity of 1,3-propanediol by Clostridium butyricum JKT37 and the role of using calcium carbonate as pH neutraliser in glycerol fermentation. / Tee, Zhao Kang; Md Jahim, Jamaliah; Tan, Jian Ping; Kim, Byung Hong.

In: Bioresource Technology, Vol. 233, 2017, p. 296-304.

Research output: Contribution to journalArticle

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