Predominance of G to A codon 12 mutation K-ras gene in Dukes' B colorectal cancer

O. Zulhabri, A. Rahman A. Jamal, Ismail Sagap, M. R. Isa, W. N. Wan Zurinah

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Introduction K-ras gene mutations in codons 12 and 13 are one of the earliest events in colon carcinogenesis. Method s DNA was extracted from 25 mg of tumour tissue (n = 70) that were taken from tumour mass and pairs with normal epithelial tissue distant from the tumour of colorectal cancer patients. Exon 1 and exon 2 of the K-ras gene were amplified. Hotspot mutations were detected using polymerase chain reaction-based single-strand conformation polymorphism method and confirmed by direct DNA sequencing analysis. Results Mutations were identified in 14 out of the 70 (20%) colorectal carcinoma tissues. Single-base transition from GGT to GAT (glycine to aspartate) in codon 12 was detected in nine samples, while three samples presented with GGC to GAC transition in codon 13. Patients with large adenoma had a 12-fold higher likelihood of K-ras mutations (odds ratios [OR] 12.31; 95% confidence intervals [CI] 1.81-83.76). Tumours located at the left colon were more likely to present with K-ras mutations (OR 4.54; 95% CI 0.96-21.54). Conc lusion Our study showed a high frequency of G to A transition of codon 12 mutation of the K-ras gene, with significant correlation with tumour size and tumour location.

Original languageEnglish
Pages (from-to)26-31
Number of pages6
JournalSingapore Medical Journal
Volume53
Issue number1
Publication statusPublished - Jan 2012

Fingerprint

ras Genes
Codon
Colorectal Neoplasms
Mutation
Neoplasms
Exons
Colon
Odds Ratio
Confidence Intervals
DNA Sequence Analysis
Aspartic Acid
Adenoma
Glycine
Carcinogenesis
Epithelium
Polymerase Chain Reaction
DNA

Keywords

  • Codon 12
  • Colorectal cancer
  • Dukes' B
  • K-ras gene
  • Mutation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Predominance of G to A codon 12 mutation K-ras gene in Dukes' B colorectal cancer. / Zulhabri, O.; A. Jamal, A. Rahman; Sagap, Ismail; Isa, M. R.; Wan Zurinah, W. N.

In: Singapore Medical Journal, Vol. 53, No. 1, 01.2012, p. 26-31.

Research output: Contribution to journalArticle

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abstract = "Introduction K-ras gene mutations in codons 12 and 13 are one of the earliest events in colon carcinogenesis. Method s DNA was extracted from 25 mg of tumour tissue (n = 70) that were taken from tumour mass and pairs with normal epithelial tissue distant from the tumour of colorectal cancer patients. Exon 1 and exon 2 of the K-ras gene were amplified. Hotspot mutations were detected using polymerase chain reaction-based single-strand conformation polymorphism method and confirmed by direct DNA sequencing analysis. Results Mutations were identified in 14 out of the 70 (20{\%}) colorectal carcinoma tissues. Single-base transition from GGT to GAT (glycine to aspartate) in codon 12 was detected in nine samples, while three samples presented with GGC to GAC transition in codon 13. Patients with large adenoma had a 12-fold higher likelihood of K-ras mutations (odds ratios [OR] 12.31; 95{\%} confidence intervals [CI] 1.81-83.76). Tumours located at the left colon were more likely to present with K-ras mutations (OR 4.54; 95{\%} CI 0.96-21.54). Conc lusion Our study showed a high frequency of G to A transition of codon 12 mutation of the K-ras gene, with significant correlation with tumour size and tumour location.",
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N2 - Introduction K-ras gene mutations in codons 12 and 13 are one of the earliest events in colon carcinogenesis. Method s DNA was extracted from 25 mg of tumour tissue (n = 70) that were taken from tumour mass and pairs with normal epithelial tissue distant from the tumour of colorectal cancer patients. Exon 1 and exon 2 of the K-ras gene were amplified. Hotspot mutations were detected using polymerase chain reaction-based single-strand conformation polymorphism method and confirmed by direct DNA sequencing analysis. Results Mutations were identified in 14 out of the 70 (20%) colorectal carcinoma tissues. Single-base transition from GGT to GAT (glycine to aspartate) in codon 12 was detected in nine samples, while three samples presented with GGC to GAC transition in codon 13. Patients with large adenoma had a 12-fold higher likelihood of K-ras mutations (odds ratios [OR] 12.31; 95% confidence intervals [CI] 1.81-83.76). Tumours located at the left colon were more likely to present with K-ras mutations (OR 4.54; 95% CI 0.96-21.54). Conc lusion Our study showed a high frequency of G to A transition of codon 12 mutation of the K-ras gene, with significant correlation with tumour size and tumour location.

AB - Introduction K-ras gene mutations in codons 12 and 13 are one of the earliest events in colon carcinogenesis. Method s DNA was extracted from 25 mg of tumour tissue (n = 70) that were taken from tumour mass and pairs with normal epithelial tissue distant from the tumour of colorectal cancer patients. Exon 1 and exon 2 of the K-ras gene were amplified. Hotspot mutations were detected using polymerase chain reaction-based single-strand conformation polymorphism method and confirmed by direct DNA sequencing analysis. Results Mutations were identified in 14 out of the 70 (20%) colorectal carcinoma tissues. Single-base transition from GGT to GAT (glycine to aspartate) in codon 12 was detected in nine samples, while three samples presented with GGC to GAC transition in codon 13. Patients with large adenoma had a 12-fold higher likelihood of K-ras mutations (odds ratios [OR] 12.31; 95% confidence intervals [CI] 1.81-83.76). Tumours located at the left colon were more likely to present with K-ras mutations (OR 4.54; 95% CI 0.96-21.54). Conc lusion Our study showed a high frequency of G to A transition of codon 12 mutation of the K-ras gene, with significant correlation with tumour size and tumour location.

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