Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype

Nor F. Rajab, Beclan J. McKenna, Jim Diamond, Kate Williamson, Peter W. Hamilton, Valerie J. McKelvey-Martin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Nuclear texture analysis measures phenotypic changes in chromatin distribution within a cell nucleus, while the alkaline Comet assay is a sensitive method for measuring the extent of DNA breakage in individual cells. The authors aim to use both methods to provide information about the sensitivity of cells to ionizing radiation. Methods: The alkaline Comet assay was performed on six human bladder carcinoma cell lines and one human urothelial cell line exposed to γ-radiation doses from 0 to 10 Gy. Nuclear chromatin texture analysis of 40 features was then performed in the same cell lines exposed to 0, 2, and 6 Gy to explore if nuclear phenotype was related to radiation sensitivity. Results: Comet assay results demonstrated that the cell lines exhibited different levels of radiosensitivity and could be divided into a radiosensitive and a radioresistant group at >6 Gy. Using stepwise discriminant analysis, a subset of important nuclear texture features that best discriminated between sensitive and resistant cell lines were identified A classification function, defined using these features, correctly classified 81.75% of all cells into their radiosensitive or radioresistant groups based on their pre-treatment chromatin phenotype. Posttreatment chromatin changes also varied between cell lines, with sensitive cell lines showing a relaxed chromatin conformation following radiation, whereas resistant cell lines exhibited chromatin condensation. Conclusions: The authors conclude that the alkaline Comet assay and nuclear texture methodologies may prove to be valuable aids in predicting the response of tumor cells to radiotherapy.

Original languageEnglish
Pages (from-to)1077-1085
Number of pages9
JournalCytometry Part A
Volume69
Issue number10
DOIs
Publication statusPublished - Oct 2006
Externally publishedYes

Fingerprint

Radiation Tolerance
Chromatin
Urinary Bladder
Phenotype
Cell Line
Comet Assay
Radiation
Discriminant Analysis
Ionizing Radiation
Cell Nucleus
Radiotherapy
Carcinoma
DNA

Keywords

  • Bladder
  • Chromatin morphology
  • Comet assay
  • Nuclear texture
  • Radiosensitivity

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Rajab, N. F., McKenna, B. J., Diamond, J., Williamson, K., Hamilton, P. W., & McKelvey-Martin, V. J. (2006). Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype. Cytometry Part A, 69(10), 1077-1085. https://doi.org/10.1002/cyto.a.20329

Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype. / Rajab, Nor F.; McKenna, Beclan J.; Diamond, Jim; Williamson, Kate; Hamilton, Peter W.; McKelvey-Martin, Valerie J.

In: Cytometry Part A, Vol. 69, No. 10, 10.2006, p. 1077-1085.

Research output: Contribution to journalArticle

Rajab, NF, McKenna, BJ, Diamond, J, Williamson, K, Hamilton, PW & McKelvey-Martin, VJ 2006, 'Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype', Cytometry Part A, vol. 69, no. 10, pp. 1077-1085. https://doi.org/10.1002/cyto.a.20329
Rajab NF, McKenna BJ, Diamond J, Williamson K, Hamilton PW, McKelvey-Martin VJ. Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype. Cytometry Part A. 2006 Oct;69(10):1077-1085. https://doi.org/10.1002/cyto.a.20329
Rajab, Nor F. ; McKenna, Beclan J. ; Diamond, Jim ; Williamson, Kate ; Hamilton, Peter W. ; McKelvey-Martin, Valerie J. / Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype. In: Cytometry Part A. 2006 ; Vol. 69, No. 10. pp. 1077-1085.
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