Precise identification of different stages of a tick, Ixodes granulatus Supino, 1897 (Acari

Ixodidae)

Ernieenor Faraliana Che Lah, Salmah Yaakop, Mariana Ahamad, Ernna George, Shukor Md. Nor

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective To identify different stages of Ixodes granulatus (I. granulatus) based on morphological characters prior to molecular identification which is significant for confirming and identifying the nymphal stages of I. granulatus. Methods A total of 14 individuals of adult, engorged and nymphal ticks collected from three different localities were examined morphologically using taxonomic keys, followed by PCR using cytochrome oxidase subunit I (COI). Clustering analysis based on COI sequences was carried out by constructing neighbor-joining and maximum parsimony tree to clarify the genetic variation and diversity of local I. granulatus. Results Based on external morphological characterizations, nine individuals (64.3%) were successfully identified as I. granulatus, while five individuals were recognized only as Ixodes sp. due to lack of morphological characters visible and development during that stage. Molecular analysis of local I. granulatus using COI gene revealed 93%–94% sequence homology from available sequence in GenBank and was in concordance with the morphological identification. Furthermore, a low intraspecific variation was observed among the species of I. granulatus collected from different localities (0%–3.7%). Conclusions These findings demonstrated for the first time the establishment of COI gene for identifying I. granulatus nymphal tick which is of paramount importance to the control of potential tick-borne infections in Malaysia. Moreover, this study provides evidence that a combination of morphology and molecular data was corroborated as an accurate tool for tick identification.

Original languageEnglish
Pages (from-to)597-604
Number of pages8
JournalAsian Pacific Journal of Tropical Biomedicine
Volume6
Issue number7
DOIs
Publication statusPublished - 1 Jul 2016

Fingerprint

Ixodidae
Ixodes
Ticks
Electron Transport Complex IV
Genes
Joining
Tick-Borne Diseases
Malaysia
Nucleic Acid Databases
Sequence Homology
Cluster Analysis
Polymerase Chain Reaction

Keywords

  • Cytochrome oxidase subunit I
  • Ixodes granulatus
  • Morphology
  • Ticks

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Precise identification of different stages of a tick, Ixodes granulatus Supino, 1897 (Acari : Ixodidae). / Che Lah, Ernieenor Faraliana; Yaakop, Salmah; Ahamad, Mariana; George, Ernna; Md. Nor, Shukor.

In: Asian Pacific Journal of Tropical Biomedicine, Vol. 6, No. 7, 01.07.2016, p. 597-604.

Research output: Contribution to journalArticle

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