Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model

Dayang Marshitah Bajury, Muhamad Hanif Rawi, Iqbal Hakim Sazali, Aminah Abdullah, Shahrul Razid Sarbini

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Red seaweed (Kappaphycus alvarezii) cultivated from Sabah (RSS) and Langkawi (RSL) were digested using in vitro mouth, gastric and duodenal model. The digested seaweed then fermented in a pH-controlled batch culture system inoculated with human faeces to mimic the distal colon. Bacterial enumeration were monitored using fluorescent in situ hybridisation, and the fermentation end products, the short chain fatty acids (SCFA), were analysed using HPLC. Both RSS and RSL showed significant increase of Bifidobacterium sp.; from log10 7.96 at 0 h to log10 8.72 at 24 h, and from log10 7.96 at 0 h to log10 8.60 at 24 h, respectively, and shows no significant difference when compared to the Bifidobacterium sp. count at 24 h of inulin fermentation. Both seaweeds also showed significant increase in total SCFA production, particularly acetate and propionate. Overall, this data suggested that K. alvarezii might have the potential as a prebiotic ingredient.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalInternational Journal of Food Sciences and Nutrition
DOIs
Publication statusAccepted/In press - 5 Apr 2017

Fingerprint

Kappaphycus alvarezii
Seaweed
Prebiotics
prebiotics
colon
macroalgae
Colon
Bifidobacterium
Volatile Fatty Acids
short chain fatty acids
Fermentation
fermentation
Batch Cell Culture Techniques
Inulin
Malaysia
Propionates
inulin
Borneo
fluorescence in situ hybridization
Fluorescence In Situ Hybridization

Keywords

  • Bifidobacteria
  • colon model
  • gut microbiota
  • Prebiotic
  • seaweed

ASJC Scopus subject areas

  • Food Science

Cite this

Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model. / Bajury, Dayang Marshitah; Rawi, Muhamad Hanif; Sazali, Iqbal Hakim; Abdullah, Aminah; Sarbini, Shahrul Razid.

In: International Journal of Food Sciences and Nutrition, 05.04.2017, p. 1-8.

Research output: Contribution to journalArticle

Bajury, Dayang Marshitah ; Rawi, Muhamad Hanif ; Sazali, Iqbal Hakim ; Abdullah, Aminah ; Sarbini, Shahrul Razid. / Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model. In: International Journal of Food Sciences and Nutrition. 2017 ; pp. 1-8.
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