Pre-optimization of medium for biobutanol production by a new isolate of solvent-producing clostridium

Najeeb Kaid Al-Shorgani, Aidil Abdul Hamid, Wan Mohtar Wan Yusoff, Mohd. Sahaid Kalil

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A Plackett-Burman design was used to pre-optimize the medium composition for biobutanol production using a unique isolate of solvent-producing Clostridium YM1. Various nutrient factors affecting biobutanol production were screened using the Plackett-Burman design. These factors included: glucose, tryptone, yeast extract, peptone, ammonium acetate, KH2PO4, K2HPO4, MgSO4, FeSO4, Na2CO3, and NaCl. The results were analyzed by an analysis of variance (ANOVA), which showed that glucose, tryptone, yeast extract, peptone, K2HPO4, Na2CO3, and MgSO4 had significant effects on biobutanol production. However, ammonium acetate, KH2PO4, and FeSO4 had insignificant effects. The established model from the ANOVA analysis had a significant value of Pmodel>F = 0.0245 and an R2 value of 0.999. The estimated maximum biobutanol production was 9.01 g/L, whereas the optimized medium produced 10.93 g/L of biobutanol.

Original languageEnglish
Pages (from-to)1420-1430
Number of pages11
JournalBioResources
Volume8
Issue number1
Publication statusPublished - Feb 2013

Fingerprint

Clostridium
variance analysis
Peptones
yeast
acetate
glucose
ammonium
Analysis of variance (ANOVA)
Yeast
Glucose
Nutrients
nutrient
Chemical analysis
effect
tryptones
ammonium acetate
potassium phosphate

Keywords

  • Biobutanol production
  • Medium optimization
  • Novel butanol-producing strain
  • Plackett-Burman design

ASJC Scopus subject areas

  • Waste Management and Disposal
  • Environmental Engineering
  • Bioengineering

Cite this

Pre-optimization of medium for biobutanol production by a new isolate of solvent-producing clostridium. / Al-Shorgani, Najeeb Kaid; Abdul Hamid, Aidil; Wan Yusoff, Wan Mohtar; Kalil, Mohd. Sahaid.

In: BioResources, Vol. 8, No. 1, 02.2013, p. 1420-1430.

Research output: Contribution to journalArticle

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