Potential of Human Decidua Stem Cells for Angiogenesis and Neurogenesis

Abdul Rahman Hayati, Mohd Manzor Nur Fariha, Geok Chin Tan, Ay Eeng Tan, Chua Kien Hui

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background and Aims: Placenta as a fetomaternal organ is a potential source of fetal as well as maternal stem cells. This present study describes novel properties of the cells isolated from the maternal part of term placenta membrane, the decidua basalis. Methods: Colony-forming unit-fibroblast (CFU-F) frequency and immunophenotype of human decidua-derived stem cells (hDSC) was carried out using flow cytometry. Quantitative polymerase chain reaction was performed to reveal the stemness, angiogenic- and endothelial cell-associated genes expression in serial-passage hDSC. Adipogenic and osteogenic differentiation potential of passage 5 (P5) cells were determined. We also performed immunostaining of common angiogenic/endogenic (CD31 and vWF) and neurogenic markers (GFAP, NF, NSE, vimentin and nestin) on hDSC at P5. Results: HDSC contains high clonogenic precursor with 1:25 CFU-F frequency. Mesenchymal stem cell-associated markers CD90, CD9, CD44, CD73 and HLA ABC were highly expressed in P0 and P5 hDSC. The specific lineage markers CD117, CD45, CD34, CD31 and HLA DR DP DQ were scarcely expressed. HDSC expressed all the stem cell-associated genes and the expression was maintained until P5. Also, the cells are capable of differentiating into adipogenic and osteogenic lineage. Positive expressions of angiogenic/endogenic markers (CD31, vWF) and neurogenic markers (GFAP, NF, NSE, vimentin and nestin) were demonstrated by hDSC. Conclusions: Human decidua contains stem cells with great proliferation capacity and mesenchymal properties. Expressions of angiogenic/endogenic and neurogenic markers support the conclusion that hDSC is a promising stem cell source for neurogenesis as well as angiogenesis.

Original languageEnglish
Pages (from-to)291-300
Number of pages10
JournalArchives of Medical Research
Volume42
Issue number4
DOIs
Publication statusPublished - May 2011

Fingerprint

Decidua
Neurogenesis
Stem Cells
Nestin
Vimentin
Placenta
Fibroblasts
HLA-DP Antigens
Mothers
Serial Passage
Gene Expression
HLA-DR Antigens
Mesenchymal Stromal Cells
Flow Cytometry
Endothelial Cells

Keywords

  • Angiogenesis
  • Flow cytometry
  • Neurogenesis
  • Placenta stem cells
  • Quantitative gene expression
  • Serial-passage
  • Stemness

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Potential of Human Decidua Stem Cells for Angiogenesis and Neurogenesis. / Hayati, Abdul Rahman; Nur Fariha, Mohd Manzor; Tan, Geok Chin; Tan, Ay Eeng; Kien Hui, Chua.

In: Archives of Medical Research, Vol. 42, No. 4, 05.2011, p. 291-300.

Research output: Contribution to journalArticle

Hayati, Abdul Rahman ; Nur Fariha, Mohd Manzor ; Tan, Geok Chin ; Tan, Ay Eeng ; Kien Hui, Chua. / Potential of Human Decidua Stem Cells for Angiogenesis and Neurogenesis. In: Archives of Medical Research. 2011 ; Vol. 42, No. 4. pp. 291-300.
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