Potential of hexavalent chromium-resistant rhizosphere bacteria in promoting plant growth and hexavalent chromium reduction

Mohd A. Kamaruzzaman, Siti Rozaimah Sheikh Abdullah, Hassimi Abu Hasan, M. Hassan, Mushrifah Idris, Nur Izzati Ismail

Research output: Contribution to journalArticle

Abstract

Aim: This research aimed to evaluate three Cr(VI)-resistant rhizosphere bacteria (Bacillus cereus, Bacillus aerius and Exiguobacterium profundum) for their ability to produce plant growth-promoting (PGP) substances and to remove Cr(VI). Methodology: Three rhizosphere bacteria were characterized for their ability to produce several PGP substances, including ammonia and indole-3-acetic acid (IAA) and solubilized phosphate. The Cr(VI) reduction ability of the rhizosphere bacteria was determined by diphenylcarbazide method with 60 mg l-1 Cr(VI). Results: Bioreduction of Cr(VI) in the reduction media by B. cereus was 13.7%, which was higher compared with those of B. aerius (4.4%) and E. profundum (3.6%). B. aerius adsorbed high Cr(VI) concentration of 56.1 mg g-1. The best condition for these rhizobacteria to remove or adsorb Cr(VI) was at acidic pH (5.36-5.97). All rhizobacteria could not stand the toxic effect of Cr(VI) at 60 mg l-1, which decreased almost 100% of rhizobacteria growth. B. cereus and B. aerius produced PGP substances, including ammonia and IAA and solubilized phosphate. Interpretation: B. cereus and B. aerius with high PGP activities can be considered promising agents in microbe-assisted phytoremediation. All rhizosphere bacteria tolerated Cr(VI) and protected plants against the inhibitory effect of Cr(VI) by reducing Cr(VI) to Cr(III) and assisting the plant uptake of Cr(III).

Original languageEnglish
Pages (from-to)427-433
Number of pages7
JournalJournal of Environmental Biology
Volume40
Issue number3
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Rhizosphere
Bacillus cereus
Chromium
Bacteria
Growth
Growth Substances
Acetic acid
Ammonia
Phosphates
Bacilli
Diphenylcarbazide
chromium hexavalent ion
Environmental Biodegradation
Poisons
Bacillus
desloratadine

Keywords

  • Bioreduction
  • Hexavalent chromium
  • Plant growth-promoting rhizobacteria
  • Rhizobacteria
  • Scirpus grossus

ASJC Scopus subject areas

  • Environmental Engineering
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Potential of hexavalent chromium-resistant rhizosphere bacteria in promoting plant growth and hexavalent chromium reduction. / Kamaruzzaman, Mohd A.; Sheikh Abdullah, Siti Rozaimah; Abu Hasan, Hassimi; Hassan, M.; Idris, Mushrifah; Ismail, Nur Izzati.

In: Journal of Environmental Biology, Vol. 40, No. 3, 01.01.2019, p. 427-433.

Research output: Contribution to journalArticle

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abstract = "Aim: This research aimed to evaluate three Cr(VI)-resistant rhizosphere bacteria (Bacillus cereus, Bacillus aerius and Exiguobacterium profundum) for their ability to produce plant growth-promoting (PGP) substances and to remove Cr(VI). Methodology: Three rhizosphere bacteria were characterized for their ability to produce several PGP substances, including ammonia and indole-3-acetic acid (IAA) and solubilized phosphate. The Cr(VI) reduction ability of the rhizosphere bacteria was determined by diphenylcarbazide method with 60 mg l-1 Cr(VI). Results: Bioreduction of Cr(VI) in the reduction media by B. cereus was 13.7{\%}, which was higher compared with those of B. aerius (4.4{\%}) and E. profundum (3.6{\%}). B. aerius adsorbed high Cr(VI) concentration of 56.1 mg g-1. The best condition for these rhizobacteria to remove or adsorb Cr(VI) was at acidic pH (5.36-5.97). All rhizobacteria could not stand the toxic effect of Cr(VI) at 60 mg l-1, which decreased almost 100{\%} of rhizobacteria growth. B. cereus and B. aerius produced PGP substances, including ammonia and IAA and solubilized phosphate. Interpretation: B. cereus and B. aerius with high PGP activities can be considered promising agents in microbe-assisted phytoremediation. All rhizosphere bacteria tolerated Cr(VI) and protected plants against the inhibitory effect of Cr(VI) by reducing Cr(VI) to Cr(III) and assisting the plant uptake of Cr(III).",
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AU - Idris, Mushrifah

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