Potential molecular signatures in epithelial ovarian cancer by genome wide expression profiling

Yin Ling Wong, Ahmad Zailani Hatta Mohd Dali, Isa Mohamed Rose, A. Rahman A. Jamal, Norfilza Mohd Mokhtar

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aims: Ovarian cancer is the deadliest of all gynecologic cancers because of its late diagnosis and poor treatment outcomes. This study aimed to identify potential molecular signatures associated with biological processes that are implicated in epithelial ovarian cancer (EOC). Methods: Expression profiling was carried out on 16 fresh frozen EOC and normal ovarian tissue samples using the Illumina Whole Genome DASL assay (cDNA-mediated annealing, selection, extension and ligation). The differentially expressed genes were analyzed using the GeneSpring GX11.5 and Pathway Studio 8.0 software. The microarray results were validated using the immunohistochemistry analyses. Results: Unpaired t-test identified 652 (270 up- and 382 downregulated) significant differentially expressed genes (P < 0.001 and fold change ≥2.0). Hierarchical clustering analysis displayed a distinct separation of cancer and normal samples. Gene set enrichment analysis identified alterations in the expression of genes associated with cancer development and progression. Positive immunostaining of claudin-7, ephrin receptor A1 and Forkhead Box M1 in EOC was consistent with the upregulation of these genes in the microarray result. However, the positive immunostaining of fibroblast growth factor-7 in cancer tissues was not in accordance with the downregulation of this gene in the microarray result. Conclusion: These results identify significant genes and their related biological processes which may contribute to the better understanding of development and progression of epithelial ovarian cancer.

Original languageEnglish
Pages (from-to)e259-e268
JournalAsia-Pacific Journal of Clinical Oncology
Volume12
Issue number2
DOIs
Publication statusPublished - 1 Jun 2016

Fingerprint

Genome
Genes
Biological Phenomena
Ephrin-A1
Neoplasms
Down-Regulation
Eph Family Receptors
Fibroblast Growth Factor 7
Delayed Diagnosis
Ovarian Neoplasms
Ligation
Cluster Analysis
Ovarian epithelial cancer
Up-Regulation
Software
Complementary DNA
Immunohistochemistry
Gene Expression

Keywords

  • biological process
  • epithelial ovarian cancer
  • gene expression profiling
  • immunohistochemistry
  • microarray

ASJC Scopus subject areas

  • Oncology

Cite this

Potential molecular signatures in epithelial ovarian cancer by genome wide expression profiling. / Wong, Yin Ling; Dali, Ahmad Zailani Hatta Mohd; Mohamed Rose, Isa; A. Jamal, A. Rahman; Mohd Mokhtar, Norfilza.

In: Asia-Pacific Journal of Clinical Oncology, Vol. 12, No. 2, 01.06.2016, p. e259-e268.

Research output: Contribution to journalArticle

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