Keupayaan pembezaan tiga Jenis sel primitif daripada hasil perbezaan tempoh proliferasi darah mencit

Translated title of the contribution: Potential differentiation of three types of primitive cells originated from different proliferation terms of mouse blood

Intan Zarina Zainol Abidin, Shahrul Hisham Zainal Ariffin, Zaidah Zainal Ariffin, Rohaya Megat Abdul Wahab

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The aim of this study was to differentiate central blood system mononucleated cells in vitro into osteoblast and osteoclast cells for three different proliferation terms of cells. The mononucleated cells were cultured in a selective proliferation medium for three different proliferation terms, i.e., short (5 days), medium (15 days) and long term (30 days) prior to analysis of osteoblast and osteoclast cells 'differentiation potentialities. The proliferation medium was then supplemented with differentiation factors, i.e., ascorbic acid and β-glycerophosphate to differentiate mononucleated cells into osteoblast cells. For osteoclast assay, RANKL and M-CSF were added into proliferation medium. For control, the same cells were used without supplementation of respective differentiation factors. The viability of differentiated cells from short, medium and long types of cells showed that they were able to survive until 10 to 14 days in the presence of respective differentiation factors without significant increased in the specific differentiation medium. Biochemical analyses on both alkaline phosphatase (ALP) and tartrate resistant acid phosphatase (TRAP) activities were significantly increased (p<0.05) once cultured in their respective differentiation medium. In conclusion, the three types of primitive cells have the same potentiality to differentiate into mature osteoblast and osteoclast cells even though the proliferation rates are different, i.e. 0.37, 0.55 and 0.72 division/day for short, medium and long term cells respectively. Mononucleated cells isolated from peripheral blood are primitive enough to differentiate into two distinct types of mature cells which originated from two different stem cells lineage hence can be categorized as multipotent stem cells.

Original languageUndefined/Unknown
Pages (from-to)305-313
Number of pages9
JournalSains Malaysiana
Volume39
Issue number2
Publication statusPublished - Apr 2010

Fingerprint

blood
mice
osteoclasts
osteoblasts
cells
stem cells
acid phosphatase
cell differentiation
cell viability
alkaline phosphatase
ascorbic acid
assays

Keywords

  • Mice
  • Osteoblast
  • Osteoclast
  • Peripheral blood
  • Stem cells

ASJC Scopus subject areas

  • General

Cite this

Keupayaan pembezaan tiga Jenis sel primitif daripada hasil perbezaan tempoh proliferasi darah mencit. / Abidin, Intan Zarina Zainol; Zainal Ariffin, Shahrul Hisham; Ariffin, Zaidah Zainal; Megat Abdul Wahab, Rohaya.

In: Sains Malaysiana, Vol. 39, No. 2, 04.2010, p. 305-313.

Research output: Contribution to journalArticle

@article{174248ffb2b4476c8ad208bdc5e3d593,
title = "Keupayaan pembezaan tiga Jenis sel primitif daripada hasil perbezaan tempoh proliferasi darah mencit",
abstract = "The aim of this study was to differentiate central blood system mononucleated cells in vitro into osteoblast and osteoclast cells for three different proliferation terms of cells. The mononucleated cells were cultured in a selective proliferation medium for three different proliferation terms, i.e., short (5 days), medium (15 days) and long term (30 days) prior to analysis of osteoblast and osteoclast cells 'differentiation potentialities. The proliferation medium was then supplemented with differentiation factors, i.e., ascorbic acid and β-glycerophosphate to differentiate mononucleated cells into osteoblast cells. For osteoclast assay, RANKL and M-CSF were added into proliferation medium. For control, the same cells were used without supplementation of respective differentiation factors. The viability of differentiated cells from short, medium and long types of cells showed that they were able to survive until 10 to 14 days in the presence of respective differentiation factors without significant increased in the specific differentiation medium. Biochemical analyses on both alkaline phosphatase (ALP) and tartrate resistant acid phosphatase (TRAP) activities were significantly increased (p<0.05) once cultured in their respective differentiation medium. In conclusion, the three types of primitive cells have the same potentiality to differentiate into mature osteoblast and osteoclast cells even though the proliferation rates are different, i.e. 0.37, 0.55 and 0.72 division/day for short, medium and long term cells respectively. Mononucleated cells isolated from peripheral blood are primitive enough to differentiate into two distinct types of mature cells which originated from two different stem cells lineage hence can be categorized as multipotent stem cells.",
keywords = "Mice, Osteoblast, Osteoclast, Peripheral blood, Stem cells",
author = "Abidin, {Intan Zarina Zainol} and {Zainal Ariffin}, {Shahrul Hisham} and Ariffin, {Zaidah Zainal} and {Megat Abdul Wahab}, Rohaya",
year = "2010",
month = "4",
language = "Undefined/Unknown",
volume = "39",
pages = "305--313",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "2",

}

TY - JOUR

T1 - Keupayaan pembezaan tiga Jenis sel primitif daripada hasil perbezaan tempoh proliferasi darah mencit

AU - Abidin, Intan Zarina Zainol

AU - Zainal Ariffin, Shahrul Hisham

AU - Ariffin, Zaidah Zainal

AU - Megat Abdul Wahab, Rohaya

PY - 2010/4

Y1 - 2010/4

N2 - The aim of this study was to differentiate central blood system mononucleated cells in vitro into osteoblast and osteoclast cells for three different proliferation terms of cells. The mononucleated cells were cultured in a selective proliferation medium for three different proliferation terms, i.e., short (5 days), medium (15 days) and long term (30 days) prior to analysis of osteoblast and osteoclast cells 'differentiation potentialities. The proliferation medium was then supplemented with differentiation factors, i.e., ascorbic acid and β-glycerophosphate to differentiate mononucleated cells into osteoblast cells. For osteoclast assay, RANKL and M-CSF were added into proliferation medium. For control, the same cells were used without supplementation of respective differentiation factors. The viability of differentiated cells from short, medium and long types of cells showed that they were able to survive until 10 to 14 days in the presence of respective differentiation factors without significant increased in the specific differentiation medium. Biochemical analyses on both alkaline phosphatase (ALP) and tartrate resistant acid phosphatase (TRAP) activities were significantly increased (p<0.05) once cultured in their respective differentiation medium. In conclusion, the three types of primitive cells have the same potentiality to differentiate into mature osteoblast and osteoclast cells even though the proliferation rates are different, i.e. 0.37, 0.55 and 0.72 division/day for short, medium and long term cells respectively. Mononucleated cells isolated from peripheral blood are primitive enough to differentiate into two distinct types of mature cells which originated from two different stem cells lineage hence can be categorized as multipotent stem cells.

AB - The aim of this study was to differentiate central blood system mononucleated cells in vitro into osteoblast and osteoclast cells for three different proliferation terms of cells. The mononucleated cells were cultured in a selective proliferation medium for three different proliferation terms, i.e., short (5 days), medium (15 days) and long term (30 days) prior to analysis of osteoblast and osteoclast cells 'differentiation potentialities. The proliferation medium was then supplemented with differentiation factors, i.e., ascorbic acid and β-glycerophosphate to differentiate mononucleated cells into osteoblast cells. For osteoclast assay, RANKL and M-CSF were added into proliferation medium. For control, the same cells were used without supplementation of respective differentiation factors. The viability of differentiated cells from short, medium and long types of cells showed that they were able to survive until 10 to 14 days in the presence of respective differentiation factors without significant increased in the specific differentiation medium. Biochemical analyses on both alkaline phosphatase (ALP) and tartrate resistant acid phosphatase (TRAP) activities were significantly increased (p<0.05) once cultured in their respective differentiation medium. In conclusion, the three types of primitive cells have the same potentiality to differentiate into mature osteoblast and osteoclast cells even though the proliferation rates are different, i.e. 0.37, 0.55 and 0.72 division/day for short, medium and long term cells respectively. Mononucleated cells isolated from peripheral blood are primitive enough to differentiate into two distinct types of mature cells which originated from two different stem cells lineage hence can be categorized as multipotent stem cells.

KW - Mice

KW - Osteoblast

KW - Osteoclast

KW - Peripheral blood

KW - Stem cells

UR - http://www.scopus.com/inward/record.url?scp=77952190496&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952190496&partnerID=8YFLogxK

M3 - Article

VL - 39

SP - 305

EP - 313

JO - Sains Malaysiana

JF - Sains Malaysiana

SN - 0126-6039

IS - 2

ER -