Postharvest analysis of lowland transgenic tomato fruits harboring hpRNAi- ACO1 construct

Bita Behboodian, Zainon Mohd Ali, Ismanizan Ismail, Zamri Zainal

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The plant hormone, ethylene, is an important regulator which involved in regulating fruit ripening and flower senescence. In this study, RNA interference (RNAi) technology was employed to silence the genes involved in ethylene biosynthetic pathway. This was achieved by blocking the expression of specific gene encoding the ACC oxidase. Initially, cDNA corresponding to ACO1 of lowland tomato cultivar (MT1), which has high identity with ACO1 of Solanum lycopersicum in GenBank, was cloned through RT-PCR. Using a partial coding region of ACO1, one hpRNAi transformation vector was constructed and expressed ectopically under the 35S promoter. Results showed that transgenic lines harboring the hpRNA-ACO1 construct had lower ethylene production and a longer shelf life of 32 days as compared to 10 days for wild-type fruits. Changes in cell wall degrading enzyme activities were also investigated in cases where the transgenic fruits exhibited reduced rates of firmness loss, which can be associated with a decrease in pectin methylesterase (PME) and polygalacturonase (PG) activities. However, no significant change was detected in both transgenic and wild-type fruits in terms of β-galactosidase (β-Gal) activity and levels of total soluble solid, titratable acid and ascorbic acid.

Original languageEnglish
Article number439870
JournalThe Scientific World Journal
Volume2012
DOIs
Publication statusPublished - 2012

Fingerprint

Lycopersicon esculentum
Fruits
Fruit
fruit
ethylene
Galactosidases
Polygalacturonase
Gene encoding
Plant Growth Regulators
gene
ripening
Biosynthetic Pathways
Nucleic Acid Databases
Enzyme activity
ascorbic acid
senescence
RNA Interference
Cell Wall
Ascorbic Acid
hormone

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Postharvest analysis of lowland transgenic tomato fruits harboring hpRNAi- ACO1 construct. / Behboodian, Bita; Mohd Ali, Zainon; Ismail, Ismanizan; Zainal, Zamri.

In: The Scientific World Journal, Vol. 2012, 439870, 2012.

Research output: Contribution to journalArticle

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