Post-transcriptional regulation of microRNAs in cancer: From prediction to validation

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

MicroRNA (miRNA) is a small non-coding RNA with an established function to regulate genes at the post-transcriptional level leading to suppression or degradation of its messenger RNA expression (mRNA). Its dysregulation plays a vital role in a variety of biological and pathological processes including cancer. A lot of algorithms have been established to predict the target sites of miRNA, but experimentally identifying and validating its target region is still lacking. Guidance in experimental procedures is really needed to find genuine miRNA targets. Therefore, in this review, we provide an outline on the workflow in predicting and validating the targeted sites of miRNA using several methods as a guideline for the scientists. The final outcome of this type of experiment is essential to explore the major impact of miRNA-mRNA interaction involved in the biological processes and to assist miRNA-based drug development in the future.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalOncology Reviews
Volume12
Issue number1
DOIs
Publication statusPublished - 30 Jan 2018

Fingerprint

MicroRNAs
Biological Phenomena
Neoplasms
Small Untranslated RNA
Messenger RNA
Workflow
Pathologic Processes
Guidelines
Pharmaceutical Preparations
Genes

Keywords

  • Algorithm
  • Cancer
  • MicroRNA
  • Target identification

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

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title = "Post-transcriptional regulation of microRNAs in cancer: From prediction to validation",
abstract = "MicroRNA (miRNA) is a small non-coding RNA with an established function to regulate genes at the post-transcriptional level leading to suppression or degradation of its messenger RNA expression (mRNA). Its dysregulation plays a vital role in a variety of biological and pathological processes including cancer. A lot of algorithms have been established to predict the target sites of miRNA, but experimentally identifying and validating its target region is still lacking. Guidance in experimental procedures is really needed to find genuine miRNA targets. Therefore, in this review, we provide an outline on the workflow in predicting and validating the targeted sites of miRNA using several methods as a guideline for the scientists. The final outcome of this type of experiment is essential to explore the major impact of miRNA-mRNA interaction involved in the biological processes and to assist miRNA-based drug development in the future.",
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author = "Ankasha, {Sheril June} and Shafiee, {Mohamad Nasir} and {Abdul Wahab}, Norhazlina and {Raja Ali}, {Raja Affendi} and {Mohd Mokhtar}, Norfilza",
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T2 - From prediction to validation

AU - Ankasha, Sheril June

AU - Shafiee, Mohamad Nasir

AU - Abdul Wahab, Norhazlina

AU - Raja Ali, Raja Affendi

AU - Mohd Mokhtar, Norfilza

PY - 2018/1/30

Y1 - 2018/1/30

N2 - MicroRNA (miRNA) is a small non-coding RNA with an established function to regulate genes at the post-transcriptional level leading to suppression or degradation of its messenger RNA expression (mRNA). Its dysregulation plays a vital role in a variety of biological and pathological processes including cancer. A lot of algorithms have been established to predict the target sites of miRNA, but experimentally identifying and validating its target region is still lacking. Guidance in experimental procedures is really needed to find genuine miRNA targets. Therefore, in this review, we provide an outline on the workflow in predicting and validating the targeted sites of miRNA using several methods as a guideline for the scientists. The final outcome of this type of experiment is essential to explore the major impact of miRNA-mRNA interaction involved in the biological processes and to assist miRNA-based drug development in the future.

AB - MicroRNA (miRNA) is a small non-coding RNA with an established function to regulate genes at the post-transcriptional level leading to suppression or degradation of its messenger RNA expression (mRNA). Its dysregulation plays a vital role in a variety of biological and pathological processes including cancer. A lot of algorithms have been established to predict the target sites of miRNA, but experimentally identifying and validating its target region is still lacking. Guidance in experimental procedures is really needed to find genuine miRNA targets. Therefore, in this review, we provide an outline on the workflow in predicting and validating the targeted sites of miRNA using several methods as a guideline for the scientists. The final outcome of this type of experiment is essential to explore the major impact of miRNA-mRNA interaction involved in the biological processes and to assist miRNA-based drug development in the future.

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