Portable Polymerase Chain Reaction (PCR): Thermal ramping rate performance evaluation

K. S. Chong, Gan Kok Beng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Thermal ramping rate is the key parameters to determine the performance of portable PCR. In this work, a portable PCR system was developed with two different dimensions of Aluminium block. The dimensions of the blocks were 30 mm × 30 mm × 8 mm (portable-8) and 30 mm × 30 mm × 15 mm (portable-15). The thermal ramping rate of the block was evaluated by heating up and cooling down the block between 50°C to 95°C. Commercial portable PCR systems like OpenPCR, Slim PCR cycler and miniPCR™ were used as a benchmark to determine the thermal ramping rate of the developed system. The results show the portable-8 had better thermal ramping rate performance and shorter heating period.

Original languageEnglish
Title of host publicationIECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages447-449
Number of pages3
ISBN (Electronic)9781467377911
DOIs
Publication statusPublished - 3 Feb 2017
Externally publishedYes
Event2016 IEEE-EMBS Conference on Biomedical Engineering and Sciences, IECBES 2016 - Kuala Lumpur, Malaysia
Duration: 4 Dec 20168 Dec 2016

Other

Other2016 IEEE-EMBS Conference on Biomedical Engineering and Sciences, IECBES 2016
CountryMalaysia
CityKuala Lumpur
Period4/12/168/12/16

Fingerprint

polymerase chain reaction
Polymerase chain reaction
evaluation
Heating
heating
Cooling
aluminum
Aluminum
cooling
Hot Temperature

Keywords

  • Polymerase Chain Reaction (PCR)
  • Temperature uniformity
  • Thermal ramping rate

ASJC Scopus subject areas

  • Biomedical Engineering
  • Instrumentation

Cite this

Chong, K. S., & Kok Beng, G. (2017). Portable Polymerase Chain Reaction (PCR): Thermal ramping rate performance evaluation. In IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences (pp. 447-449). [7843490] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/IECBES.2016.7843490

Portable Polymerase Chain Reaction (PCR) : Thermal ramping rate performance evaluation. / Chong, K. S.; Kok Beng, Gan.

IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences. Institute of Electrical and Electronics Engineers Inc., 2017. p. 447-449 7843490.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chong, KS & Kok Beng, G 2017, Portable Polymerase Chain Reaction (PCR): Thermal ramping rate performance evaluation. in IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences., 7843490, Institute of Electrical and Electronics Engineers Inc., pp. 447-449, 2016 IEEE-EMBS Conference on Biomedical Engineering and Sciences, IECBES 2016, Kuala Lumpur, Malaysia, 4/12/16. https://doi.org/10.1109/IECBES.2016.7843490
Chong KS, Kok Beng G. Portable Polymerase Chain Reaction (PCR): Thermal ramping rate performance evaluation. In IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences. Institute of Electrical and Electronics Engineers Inc. 2017. p. 447-449. 7843490 https://doi.org/10.1109/IECBES.2016.7843490
Chong, K. S. ; Kok Beng, Gan. / Portable Polymerase Chain Reaction (PCR) : Thermal ramping rate performance evaluation. IECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 447-449
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