Porous three dimensional (3-D) scaffolds of poly(3-hydroxybutyric acid) (PHB) and poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) (PHBV): Determination of salt leaching efficiency of solvent-casting particulate-leaching (SCPL)

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

As a family of biodegradable polyesters, polyhydroxyalkanoates (PHAs) have attracted increasing interest as tissue engineering materials due to their general biodegradability, good biocompatibility, together with other adjustable mechanical properties. Among PHA, polyhydroxybutyrate (PHB) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) were selected for the fabrication of porous 3-D scaffolds. The scaffolds of poly(3-hydroxybutyric acid) (PHB) and poly(3-hydroxybutyric acid-co-3- hydroxyvaleric acid) (PHBV) with an improved thickness greater than 1 mm were fabricated using a conventional method of solvent-casting particulate-leaching (SCPL). The polymeric porous 3-D scaffolds were fabricated using an ideal polymer concentration of 4% (w/v). Even since the technique introduced in the 90's, to our knowledge, there was no studies have been conducted to measure the efficiency of the salt leaching process. The objectives of this study are to determine the amount of salt remains inside the porous 3-D scaffolds and the effect of salt remnants on cell growth media electrolytes content by using the conductivity (κ) measurement. Based on these findings, both polymeric porous 3-D scaffolds with an improved thickness did not possess any effects to the electrolytes balance inside the cell growth media which could possibly lead to cellular damage (e.g., necrosis). Therefore, it has been proven that this method was undoubtedly the suitable method to construct porous 3-D scaffolds with an improved thickness greater than 1 mm without perturbing to any contaminants (e.g., porogen and solvents) that could affect the in vitro cell proliferation and tissue growth.

Original languageEnglish
Pages (from-to)925-932
Number of pages8
JournalAdvances in Environmental Biology
Volume8
Issue number10
Publication statusPublished - 2014

Fingerprint

polyhydroxybutyrate
3-hydroxybutyric acid
Hydroxybutyrates
Polyhydroxyalkanoates
leaching
particulates
Salts
polyhydroxyalkanoates
salt
salts
electrolyte
biodegradability
acids
acid
Growth
electrolytes
cell growth
Polyesters
Water-Electrolyte Balance
culture media

Keywords

  • 3-D scaffolds
  • Conductivity
  • PHB
  • PHBV
  • Salt leaching efficiency
  • Solventcasting particulate-leaching

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

@article{dee9a76caa224d25bbd9c8aa62d7f494,
title = "Porous three dimensional (3-D) scaffolds of poly(3-hydroxybutyric acid) (PHB) and poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) (PHBV): Determination of salt leaching efficiency of solvent-casting particulate-leaching (SCPL)",
abstract = "As a family of biodegradable polyesters, polyhydroxyalkanoates (PHAs) have attracted increasing interest as tissue engineering materials due to their general biodegradability, good biocompatibility, together with other adjustable mechanical properties. Among PHA, polyhydroxybutyrate (PHB) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) were selected for the fabrication of porous 3-D scaffolds. The scaffolds of poly(3-hydroxybutyric acid) (PHB) and poly(3-hydroxybutyric acid-co-3- hydroxyvaleric acid) (PHBV) with an improved thickness greater than 1 mm were fabricated using a conventional method of solvent-casting particulate-leaching (SCPL). The polymeric porous 3-D scaffolds were fabricated using an ideal polymer concentration of 4{\%} (w/v). Even since the technique introduced in the 90's, to our knowledge, there was no studies have been conducted to measure the efficiency of the salt leaching process. The objectives of this study are to determine the amount of salt remains inside the porous 3-D scaffolds and the effect of salt remnants on cell growth media electrolytes content by using the conductivity (κ) measurement. Based on these findings, both polymeric porous 3-D scaffolds with an improved thickness did not possess any effects to the electrolytes balance inside the cell growth media which could possibly lead to cellular damage (e.g., necrosis). Therefore, it has been proven that this method was undoubtedly the suitable method to construct porous 3-D scaffolds with an improved thickness greater than 1 mm without perturbing to any contaminants (e.g., porogen and solvents) that could affect the in vitro cell proliferation and tissue growth.",
keywords = "3-D scaffolds, Conductivity, PHB, PHBV, Salt leaching efficiency, Solventcasting particulate-leaching",
author = "Zubairi, {Saiful Irwan}",
year = "2014",
language = "English",
volume = "8",
pages = "925--932",
journal = "Advances in Environmental Biology",
issn = "1995-0756",
publisher = "American-Eurasian Network for Scientific Information",
number = "10",

}

TY - JOUR

T1 - Porous three dimensional (3-D) scaffolds of poly(3-hydroxybutyric acid) (PHB) and poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) (PHBV)

T2 - Determination of salt leaching efficiency of solvent-casting particulate-leaching (SCPL)

AU - Zubairi, Saiful Irwan

PY - 2014

Y1 - 2014

N2 - As a family of biodegradable polyesters, polyhydroxyalkanoates (PHAs) have attracted increasing interest as tissue engineering materials due to their general biodegradability, good biocompatibility, together with other adjustable mechanical properties. Among PHA, polyhydroxybutyrate (PHB) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) were selected for the fabrication of porous 3-D scaffolds. The scaffolds of poly(3-hydroxybutyric acid) (PHB) and poly(3-hydroxybutyric acid-co-3- hydroxyvaleric acid) (PHBV) with an improved thickness greater than 1 mm were fabricated using a conventional method of solvent-casting particulate-leaching (SCPL). The polymeric porous 3-D scaffolds were fabricated using an ideal polymer concentration of 4% (w/v). Even since the technique introduced in the 90's, to our knowledge, there was no studies have been conducted to measure the efficiency of the salt leaching process. The objectives of this study are to determine the amount of salt remains inside the porous 3-D scaffolds and the effect of salt remnants on cell growth media electrolytes content by using the conductivity (κ) measurement. Based on these findings, both polymeric porous 3-D scaffolds with an improved thickness did not possess any effects to the electrolytes balance inside the cell growth media which could possibly lead to cellular damage (e.g., necrosis). Therefore, it has been proven that this method was undoubtedly the suitable method to construct porous 3-D scaffolds with an improved thickness greater than 1 mm without perturbing to any contaminants (e.g., porogen and solvents) that could affect the in vitro cell proliferation and tissue growth.

AB - As a family of biodegradable polyesters, polyhydroxyalkanoates (PHAs) have attracted increasing interest as tissue engineering materials due to their general biodegradability, good biocompatibility, together with other adjustable mechanical properties. Among PHA, polyhydroxybutyrate (PHB) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) were selected for the fabrication of porous 3-D scaffolds. The scaffolds of poly(3-hydroxybutyric acid) (PHB) and poly(3-hydroxybutyric acid-co-3- hydroxyvaleric acid) (PHBV) with an improved thickness greater than 1 mm were fabricated using a conventional method of solvent-casting particulate-leaching (SCPL). The polymeric porous 3-D scaffolds were fabricated using an ideal polymer concentration of 4% (w/v). Even since the technique introduced in the 90's, to our knowledge, there was no studies have been conducted to measure the efficiency of the salt leaching process. The objectives of this study are to determine the amount of salt remains inside the porous 3-D scaffolds and the effect of salt remnants on cell growth media electrolytes content by using the conductivity (κ) measurement. Based on these findings, both polymeric porous 3-D scaffolds with an improved thickness did not possess any effects to the electrolytes balance inside the cell growth media which could possibly lead to cellular damage (e.g., necrosis). Therefore, it has been proven that this method was undoubtedly the suitable method to construct porous 3-D scaffolds with an improved thickness greater than 1 mm without perturbing to any contaminants (e.g., porogen and solvents) that could affect the in vitro cell proliferation and tissue growth.

KW - 3-D scaffolds

KW - Conductivity

KW - PHB

KW - PHBV

KW - Salt leaching efficiency

KW - Solventcasting particulate-leaching

UR - http://www.scopus.com/inward/record.url?scp=84904416550&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904416550&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84904416550

VL - 8

SP - 925

EP - 932

JO - Advances in Environmental Biology

JF - Advances in Environmental Biology

SN - 1995-0756

IS - 10

ER -