PLGA/xylitol nanoparticles enhance antibiofilm activity

Via penetration into biofilm extracellular polymeric substances

Anam Anjum, Pooi Yin Chung, Shiow Fern Ng

Research output: Contribution to journalArticle

Abstract

Biofilms are gelatinous masses of microorganisms attached to wound surfaces. Previous studies suggest that biofilms generate resistance towards antibiotic treatments. It was reported that hydrogels containing xylitol and antibiotic combinations produced additive antibiofilm inhibition. However, hydrogel formulations lack specificity, due to which xylitol cannot penetrate into the biofilm matrix and gets easily degraded by bacterial beta lactamase enzymes. It was hypothesized that the incorporation of xylitol in PLGA (polylactic-co-glycolic acid) nanoparticles will enhance penetration into the EPS (extra polymeric substance) component of the biofilm matrix and potentially overcome the antibiotic resistance associated with the biofilms. The purpose of this study was to develop PLGA nanoparticles loaded with xylitol, which will enhance bacterial biofilm penetration. The nanoparticles were loaded with different amounts of xylitol (0.5-5% w/w) and characterized for physiochemical and drug release properties. The metabolic antibiofilm activity of the PLGA nanoparticles containing xylitol was demonstrated by an XTT assay using as references the cultures of Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) and the polymicrobial biofilms of both bacterial strains. Live/dead viability staining was also performed to investigate the viability ratio of bacterial cells present in the biofilms. The biofilm penetration study of the PLGA nanoparticles was assessed by combining the nanoparticles with conjugated concanavalin A (Con A)-fluorescein isothiocyanate (FITC) and by viewing using confocal laser scanning electron microscopy (CLSM). In conclusion, the PLGA nanoparticles loaded with xylitol were successfully developed and were found to promote the antibiofilm activity of xylitol in infected wounds.

Original languageEnglish
Pages (from-to)14198-14208
Number of pages11
JournalRSC Advances
Volume9
Issue number25
DOIs
Publication statusPublished - 1 Jan 2019

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glycolic acid
Xylitol
Biofilms
Nanoparticles
Acids
Antibiotics
Anti-Bacterial Agents
Hydrogels
Hydrogel

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

PLGA/xylitol nanoparticles enhance antibiofilm activity : Via penetration into biofilm extracellular polymeric substances. / Anjum, Anam; Chung, Pooi Yin; Ng, Shiow Fern.

In: RSC Advances, Vol. 9, No. 25, 01.01.2019, p. 14198-14208.

Research output: Contribution to journalArticle

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