Plasma/serum proteomics: Depletion strategies for reducing high-abundance proteins for biomarker discovery

Pey Yee Lee, Junaida Osman, Teck Yew Low, Rahman Jamal

Research output: Contribution to journalReview article

Abstract

Plasma and serum are widely used for proteomics-based biomarker discovery. However, analysis of these biofluids is highly challenging due to the complexity and wide dynamic range of their proteomes. Notably, highly abundant proteins tend to obscure the detection of potential biomarkers that are usually of lower concentrations. Among the strategies to resolve this problem are: depletion of high-abundance proteins, enrichment of low abundant proteins of interest and prefractionation. In this review, we focus on current and emerging depletion techniques used to enhance the detection and identification of the less abundant proteins in plasma and serum. We discuss the applications and contributions of these methods to proteomics analysis of plasma and serum alongside their limitations and future perspectives.

Original languageEnglish
Pages (from-to)1799-1812
Number of pages14
JournalBioanalysis
Volume11
Issue number19
DOIs
Publication statusPublished - Oct 2019

Fingerprint

Biomarkers
Proteomics
Plasmas
Blood Proteins
Serum
Proteins
Proteome

Keywords

  • bacterial cellulose
  • biomarker
  • cibacron blue
  • combinatorial peptide ligand library (CPLL)
  • cryogels
  • depletion
  • molecular imprinted polymers
  • plasma
  • proteomics
  • serum

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

Cite this

Plasma/serum proteomics : Depletion strategies for reducing high-abundance proteins for biomarker discovery. / Lee, Pey Yee; Osman, Junaida; Low, Teck Yew; Jamal, Rahman.

In: Bioanalysis, Vol. 11, No. 19, 10.2019, p. 1799-1812.

Research output: Contribution to journalReview article

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