Plasma proteome analysis of cervical intraepithelial neoplasia and cervical squamous cell carcinoma

Mee Lee Looi, Saiful Anuar Karsani, Mariati Abdul Rahman, Ahmad Zailani Hatta Ahmad Zailani, Siti Aishah Siti, Wan Zurinah Wan Ngah, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Although cervical cancer is preventable with early detection, it remains the second most common malignancy among women. An understanding of how proteins change in their expression during a particular diseased state such as cervical cancer will contribute to an understanding of how the disease develops and progresses. Potentially, it may also lead to the ability to predict the occurrence of the disease. With this in mind, we aimed to identify differentially expressed proteins in the plasma of cervical cancer patients. Plasma from control, cervical intraepithelial neoplasia (CIN) grade 3 and squamous cell carcinoma (SCC) stage IV subjects was resolved by two-dimensional gel electrophoresis and the resulting proteome profiles compared. Differentially expressed protein spots were then identified by mass spectrometry. Eighteen proteins were found to be differentially expressed in the plasma of CIN 3 and SCC stage IV samples when compared with that of controls. Competitive ELISA further validated the expression of cytokeratin 19 and tetranectin. Functional analyses of these differentially expressed proteins will provide further insight into their potential role(s) in cervical cancer-specific monitoring and therapeutics.

Original languageEnglish
Pages (from-to)917-925
Number of pages9
JournalJournal of Biosciences
Volume34
Issue number6
DOIs
Publication statusPublished - Dec 2009

Fingerprint

Cervical Intraepithelial Neoplasia
uterine cervical neoplasms
squamous cell carcinoma
Proteome
proteome
Uterine Cervical Neoplasms
Squamous Cell Carcinoma
Plasmas
neoplasms
Proteins
proteins
Keratin-19
disease occurrence
two-dimensional gel electrophoresis
Electrophoresis, Gel, Two-Dimensional
blood proteins
Blood Proteins
Mass Spectrometry
Electrophoresis
Enzyme-Linked Immunosorbent Assay

Keywords

  • 2D electrophoresis
  • Cervical cancer
  • CIN
  • Plasma proteomics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Looi, M. L., Karsani, S. A., Abdul Rahman, M., Ahmad Zailani, A. Z. H., Siti, S. A., Ngah, W. Z. W., & Mohd Yusof, Y. A. (2009). Plasma proteome analysis of cervical intraepithelial neoplasia and cervical squamous cell carcinoma. Journal of Biosciences, 34(6), 917-925. https://doi.org/10.1007/s12038-009-0106-3

Plasma proteome analysis of cervical intraepithelial neoplasia and cervical squamous cell carcinoma. / Looi, Mee Lee; Karsani, Saiful Anuar; Abdul Rahman, Mariati; Ahmad Zailani, Ahmad Zailani Hatta; Siti, Siti Aishah; Ngah, Wan Zurinah Wan; Mohd Yusof, Yasmin Anum.

In: Journal of Biosciences, Vol. 34, No. 6, 12.2009, p. 917-925.

Research output: Contribution to journalArticle

Looi, ML, Karsani, SA, Abdul Rahman, M, Ahmad Zailani, AZH, Siti, SA, Ngah, WZW & Mohd Yusof, YA 2009, 'Plasma proteome analysis of cervical intraepithelial neoplasia and cervical squamous cell carcinoma', Journal of Biosciences, vol. 34, no. 6, pp. 917-925. https://doi.org/10.1007/s12038-009-0106-3
Looi, Mee Lee ; Karsani, Saiful Anuar ; Abdul Rahman, Mariati ; Ahmad Zailani, Ahmad Zailani Hatta ; Siti, Siti Aishah ; Ngah, Wan Zurinah Wan ; Mohd Yusof, Yasmin Anum. / Plasma proteome analysis of cervical intraepithelial neoplasia and cervical squamous cell carcinoma. In: Journal of Biosciences. 2009 ; Vol. 34, No. 6. pp. 917-925.
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