Plasma-derived microparticles in polycythaemia vera

Madzlifah Ahadon, Suria Abdul Aziz, Chieh Lee Wong, Leong Chooi Fun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Introduction: Microparticles are membrane bound vesicles, measuring less than 1.0 um, which are released during cellular activation or during apoptosis. Studies have shown that these circulating microparticles play a role in coagulation, cell signaling and cellular interactions. Increased levels of circulating microparticles have been observed in a number of conditions where there is vascular dysfunction, thrombosis and inflammation. The objective of this study was to determine the various plasma-derived microparticles in patients with polycythaemia vera (PV) in Universiti Kebangsaan Malaysia Medical Centre and to compare them with normal control. Methods: A total of 15 patients with PV and 15 healthy volunteers were included in this cross-sectional descriptive study. Plasma samples from both patients and healthy volunteers were prepared and further processed for isolation of microparticles. Flow cytometry analyses were then carried out in all samples to determine the cellular origin of the microparticles. Full blood count parameters for both groups were also collected. Data collected were analyzed using SPSS version 12.0. Results: Patients with PV had a significantly higher percentage of platelet derived microparticles compared to healthy controls (P <0.05). The control group had a higher level of endothelial derived microparticles but the differences were not statistically significant (P > 0.05). Conclusion: The median percentage of positive events for platelet derived microparticles was higher in patients with PV compared to normal healthy controls.

Original languageEnglish
Pages (from-to)41-48
Number of pages8
JournalMalaysian Journal of Pathology
Volume40
Issue number1
Publication statusPublished - 1 Apr 2018

Fingerprint

Polycythemia Vera
Healthy Volunteers
Blood Platelets
Malaysia
Blood Vessels
Flow Cytometry
Thrombosis
Cross-Sectional Studies
Apoptosis
Inflammation
Membranes

Keywords

  • Flowcytometry
  • Haemostasis
  • Microparticles
  • Polycythaemia vera

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Plasma-derived microparticles in polycythaemia vera. / Ahadon, Madzlifah; Abdul Aziz, Suria; Wong, Chieh Lee; Chooi Fun, Leong.

In: Malaysian Journal of Pathology, Vol. 40, No. 1, 01.04.2018, p. 41-48.

Research output: Contribution to journalArticle

Ahadon, Madzlifah ; Abdul Aziz, Suria ; Wong, Chieh Lee ; Chooi Fun, Leong. / Plasma-derived microparticles in polycythaemia vera. In: Malaysian Journal of Pathology. 2018 ; Vol. 40, No. 1. pp. 41-48.
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