Piper sarmentosum Effects on 11β-Hydroxysteroid Dehydrogenase Type 1 Enzyme in Serum and Bone in Rat Model of Glucocorticoid-Induced Osteoporosis

Siti Fadziyah Mohamad Asri, Elvy Suhana Mohd Ramli, Ima Nirwana Soelaiman, Muhamad Alfakry Mat Noh, Abdul Hamid Abdul Rashid, Farihah Suhaimi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Glucocorticoid-induced osteoporosis is one of the common causes of secondary osteoporosis. Piper sarmentosum (Ps) extract possesses antioxidant and anti-inflammatory activities. In this study, we determined the correlation between the effects of Ps leaf water extract with the regulation of 11β-hydroxysteroid dehydrogenase (HSD) type 1 enzyme activity in serum and bone of glucocorticoid-induced osteoporotic rats. Twenty-four Sprague-Dawley rats were grouped into following: G1: sham-operated group administered with intramuscular vehicle olive oil and vehicle normal saline orally; G2: adrenalectomized (adrx) control group given intramuscular dexamethasone (120 μg/kg/day) and vehicle normal saline orally; G3: adrx group given intramuscular dexamethasone (120 μg/kg/day) and water extract of Piper sarmentosum (125 mg/kg/day) orally. After two months, the femur and serum were taken for ELISA analysis. Results showed that Ps leaf water extract significantly reduced the femur corticosterone concentration (p < 0.05). This suggests that Ps leaf water extract was able to prevent bone loss due to long-term glucocorticoid therapy by acting locally on the bone cells by increasing the dehydrogenase action of 11β-HSD type 1. Thus, Ps may have the potential to be used as an alternative medicine against osteoporosis and osteoporotic fracture in patients on long-term glucocorticoid treatment.

Original languageEnglish
JournalMolecules (Basel, Switzerland)
Volume21
Issue number11
DOIs
Publication statusPublished - 15 Nov 2016

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glucocorticoids
11-beta-Hydroxysteroid Dehydrogenases
Piper
osteoporosis
dehydrogenases
serums
Glucocorticoids
bones
Osteoporosis
rats
enzymes
Rats
Bone
leaves
Bone and Bones
femur
vehicles
Water
Enzymes
Serum

Keywords

  • 11β-hydroxysteroid dehydrogenase
  • glucocorticoid
  • osteoporosis
  • Piper sarmentosum

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Piper sarmentosum Effects on 11β-Hydroxysteroid Dehydrogenase Type 1 Enzyme in Serum and Bone in Rat Model of Glucocorticoid-Induced Osteoporosis. / Asri, Siti Fadziyah Mohamad; Ramli, Elvy Suhana Mohd; Soelaiman, Ima Nirwana; Noh, Muhamad Alfakry Mat; Rashid, Abdul Hamid Abdul; Suhaimi, Farihah.

In: Molecules (Basel, Switzerland), Vol. 21, No. 11, 15.11.2016.

Research output: Contribution to journalArticle

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